FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005106693

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106693
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences490194
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT248585.071053501266845No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT206434.211189855444987RNA PCR Primer, Index 41 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGTCTT154253.14671334206457No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGT139572.8472400722979065No Hit
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTG126312.5767349253560834RNA PCR Primer, Index 41 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCT107852.2001493286331537RNA PCR Primer, Index 41 (95% over 24bp)
TCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGTCTTCTGCTTG94741.9327041946657852Illumina PCR Primer Index 11 (96% over 26bp)
ACACATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGTCTTCT79111.613850842727573Illumina PCR Primer Index 11 (95% over 21bp)
CATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCT58961.2027890998257833RNA PCR Primer, Index 41 (96% over 27bp)
CTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGA49611.0120482910847541RNA PCR Primer, Index 41 (96% over 29bp)
CATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGTCTTCTGCT44300.9037238317890469Illumina PCR Primer Index 11 (95% over 24bp)
CTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGTCTTCTGCTTGA37330.7615352289093706Illumina PCR Primer Index 11 (96% over 26bp)
CACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTG24150.4926620888872569TruSeq Adapter, Index 3 (95% over 24bp)
TTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC23900.48756206726316514No Hit
CACATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGTCTTCTG19820.40432971435798887RNA PCR Primer, Index 41 (95% over 23bp)
TATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCT17810.3633255405002917RNA PCR Primer, Index 41 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGTC16660.33986544102947No Hit
ACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGC12690.258877097638894RNA PCR Primer, Index 17 (96% over 25bp)
TATACACATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGTCT11350.23154098173376256No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9070.18502878452204635No Hit
ACATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGTCTTCTGC8500.17340073521911734RNA PCR Primer, Index 41 (95% over 24bp)
ATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTT7040.14361660893442188TruSeq Adapter, Index 3 (96% over 27bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5690.11607649216432676No Hit
ATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGTCTTCTGCTT5560.11342448091979909RNA PCR Primer, Index 41 (96% over 26bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCCTAAGA4910.1001644246971607No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)