FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005106701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1487718
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT71160.47831645513464244No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT56040.3766842909744992TruSeq Adapter, Index 6 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCGCGTATGCCGTCTT47710.32069249683071654No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGCGTATGCCGT41290.2775391572865288No Hit
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCT32800.22047189050613086RNA PCR Primer, Index 6 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27670.1859895490946537No Hit
ACACATCTCCGAGCCCACGAGACCGATCAGTATCGCGTATGCCGTCTTCT24630.1655555689989635RNA PCR Primer, Index 2 (95% over 21bp)
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTG23410.15735509014477206TruSeq Adapter, Index 6 (96% over 28bp)
TCTCCGAGCCCACGAGACCGATCAGTATCGCGTATGCCGTCTTCTGCTTG16710.11231967348650752TruSeq Adapter, Index 2 (96% over 26bp)
CATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCT16490.11084089861116153TruSeq Adapter, Index 6 (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16310.10963099189496935No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)