FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005106705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2245183
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT86390.38477932533784553No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT70760.315163619179372TruSeq Adapter, Index 6 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39540.17611036605924774No Hit
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCT38090.16965209517442453RNA PCR Primer, Index 6 (95% over 23bp)
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTG28390.12644848994491764TruSeq Adapter, Index 6 (96% over 28bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23310.10382227194843359No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)