FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106707

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106707
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145717
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT64294.41197663965083No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT41632.8569075674080584TruSeq Adapter, Index 6 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGCGTATGCCGT36772.523384368330394No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCGCGTATGCCGTCTT32382.2221154703981005No Hit
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCT21991.5090895365674561RNA PCR Primer, Index 6 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACCGATCAGTATCGCGTATGCCGTCTTCT17131.1755663374897918RNA PCR Primer, Index 2 (95% over 21bp)
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTG11990.8228278100702046TruSeq Adapter, Index 6 (96% over 28bp)
CATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCT9580.657438733984367TruSeq Adapter, Index 6 (96% over 26bp)
TCTCCGAGCCCACGAGACCGATCAGTATCGCGTATGCCGTCTTCTGCTTG9000.6176355538475264TruSeq Adapter, Index 2 (96% over 26bp)
CATCTCCGAGCCCACGAGACCGATCAGTATCGCGTATGCCGTCTTCTGCT7560.5188138652319221TruSeq Adapter, Index 2 (95% over 24bp)
CACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTG4970.341072078069134RNA PCR Primer, Index 40 (96% over 25bp)
TTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC4230.2902887103083374No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4120.28273983131686764No Hit
CTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTGA3690.2532305770774858RNA PCR Primer, Index 6 (96% over 28bp)
CACATCTCCGAGCCCACGAGACCGATCAGTATCGCGTATGCCGTCTTCTG3520.24156412772703253TruSeq Adapter, Index 2 (95% over 22bp)
TATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCT3130.2147999203936397No Hit
TTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGCGTATGCCGTC2900.19901590068420297No Hit
CTCCGAGCCCACGAGACCGATCAGTATCGCGTATGCCGTCTTCTGCTTGA2670.18323188097476617RNA PCR Primer, Index 2 (96% over 26bp)
CTGTAGGACCTGAAAAATGGCAAGAAAACTGAAAATCACGGAAAATGAGA2410.1653890760858376No Hit
GTCCTACAGTGTGCATTTCTCATTTTCCACGTTTTTCAGTGATTTTGTCA2310.15852645882086508No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2300.15784019709436783No Hit
TATACACATCTCCGAGCCCACGAGACCGATCAGTATCGCGTATGCCGTCT2100.1441149625644228No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCATGTTTTTCAGTGATTTCGTCA2100.1441149625644228No Hit
ACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGC1990.13656608357295305RNA PCR Primer, Index 40 (96% over 26bp)
CTGTAGGACATGGAATATGACAAGAAAAGTGAAAATCATGGAAAATGAGA1820.12489963422249978No Hit
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCG1700.11666449350453276RNA PCR Primer, Index 6 (95% over 22bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1690.1159782317780355No Hit
CTGTAGGACATGGAATATGGCAAAAAATCTGAAAATCATGGAAAATGAGA1680.11529197005153825No Hit
CTTTAGGACGTGAAATATGGCTAGGAAAACTGAAAAAGGTGGAAAATTTA1620.11117439969255474No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACTTTTTTCAGTTTTCCTCGC1550.10637056760707399No Hit
CTGTAGGACATGGAATATGGCAAAAAAACTGAAAATCATGGAAAATGAGA1500.10293925897458772No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)