FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106729

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106729
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3634516
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT174710.4806967420146176No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT140800.3873968363325406TruSeq Adapter, Index 6 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCGCGTATGCCGTCTT111870.3077988926173389No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGCGTATGCCGT100230.2757726200682567No Hit
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCT70040.19270791489155642RNA PCR Primer, Index 6 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACCGATCAGTATCGCGTATGCCGTCTTCT56120.15440845493595295RNA PCR Primer, Index 2 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50680.13944084989583208No Hit
CATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCT38160.1049933471196715TruSeq Adapter, Index 6 (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)