FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1930128
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT37810.19589374383460578RNA PCR Primer, Index 29 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT27410.14201130702212494No Hit
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCT23080.11957756169539015RNA PCR Primer, Index 29 (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21850.11320492734160637No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)