FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106747

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106747
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5386662
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT313680.5823272371646857No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT223660.41521075575189237RNA PCR Primer, Index 29 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGCGTATGCCGTCTT134870.2503776921588917No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGCGTATGCCGT133100.24709179822309252No Hit
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCT96170.17853357051175664RNA PCR Primer, Index 29 (96% over 25bp)
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA84050.1560335510191655No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76340.14172041980729438No Hit
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCGCGTATGCCGTCTTCT57010.10583548772876412Illumina PCR Primer Index 12 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)