FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3683777
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT218570.5933312467068446No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT146510.39771679990401154No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCGCGTATGCCGT109560.2974121397685039No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCGCGTATGCCGTCTT99420.26988604359058654No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCT77650.2107890895675824Illumina PCR Primer Index 7 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACTCGACGTCATCGCGTATGCCGTCTTCT52030.1412409057334361RNA PCR Primer, Index 7 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39170.10633108355907538No Hit
CATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCT37440.10163481665692575Illumina PCR Primer Index 7 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)