FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106798

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106798
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4526675
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT153180.33839407512136394No Hit
CTATTAAGCTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCT142400.3145796859725958No Hit
GCTTAATAGTGGGCCTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA134500.2971275826075431No Hit
GTATAGGAGCTACACCCCCCGGGACTACTGGGGCCAGGGAACCCTGGTCA125620.27751053477442056No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC112200.24786405032391326No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT84130.18585385520277026No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT80660.17818818448419646No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG75920.1677169224651648No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG69810.1542191564448519No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG67790.14975671988821818No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA67620.1493811682968183No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAACAG67330.1487405214644303No Hit
GATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCAGCAAGGTTCAGCG66360.14659766826644283No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC63570.1404342039134685No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC61600.13608222370724649No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA60060.13268016811456532No Hit
GTAGTGGAATGTGTGTGAGCTGGATCCGCCAGCCCCCAGGGAAGGCCCTG59800.13210579509242434No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG56910.12572141803862658No Hit
CTCCTATACTGACCACCGGCCCCCACACATAGCCGTGCACAGTAATATGT56820.12552259660788548No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC56520.12485985850541513No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA55530.12267282276726295No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC55330.12223099736561605No Hit
GTAATATGTGGCTGTGTCCACAGGGTCCATGTTGGTCATTGTGAGGACCA54940.12136943783240459No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA52790.11661981476470036No Hit
ACTTATTACTGTCAACAGCTTAATAGTGGGCCTTTCGGCCCTGGGACCAA51660.11412350124539535No Hit
CCCTCACACTGACCTGCACCTTCTCTGGGTTCTCACTCAGCAGTAGTGGA50210.11092026708345529No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT49550.10946224325802051No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA49300.10890996150596187No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA48880.10798212816250337No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC48820.10784958054200931No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT48650.10747402895060945No Hit
CTCCTGATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCAGCAAGGTT48480.10709847735920959No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC47420.10475680273048099No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGGC46840.10347550906570496No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT46150.10195121143002314No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC46050.1017302987291997No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG45750.10106756062672932No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA45730.10102337808656464No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)