FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106810

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106810
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5421569
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATATACACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGG257150.4743091898304716No Hit
GTAATACACGGCCGTGTCTTCAGATCTCAGGCTGCTCAGGTCCATGTAGA215050.3966563922731593No Hit
GTGTATATAGTACCTGGTGAAGGTGTATCCAGATGCCTTGCAGGAAACCC161600.298068695611916No Hit
GTACTATATACACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGA156520.28869871433896716No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT151120.2787384980252027No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT126990.23423108697869566No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC125510.23150124991492316No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA125160.23085568033903103No Hit
GTGTATTACTGTGCGAGAGATTATGGTTCGGGGAGTTATACAACCGACTA123340.22749871854439185No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC120070.22146725422105668No Hit
GATTATTACTGCTACTCATATGCAGGCAGCTACACTTCTGTCTTCGGAAC118320.21823940634159594No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACAGAAGTGTAGCTGC114950.21202349356800584No Hit
ATATAGTACCTGGTGAAGGTGTATCCAGATGCCTTGCAGGAAACCCTCAC113080.20857430754823927No Hit
GTCTACATGGACCTGAGCAGCCTGAGATCTGAAGACACGGCCGTGTATTA112040.20665604366558832No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA109460.20189727364901192No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG108000.19920432627529042No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG106760.1969171654921297No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC105570.19472222893409638No Hit
TTATAACTATGTCTCCTGGTACCAACAGCACCCAGCCAAAGCCCCCAAAC103850.19154971558971212No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA99890.18424555695961814No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA97710.18022458074406136No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT96990.1788965519022261No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA92320.17028280927532233No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG91740.16921300826384392No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACAGAAGTGTAGCTG91530.16882566651830863No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC90730.16735007891626943No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA88930.16403000681168126No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC87800.1619457393238009No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC87610.16159528726831662No Hit
ATATACACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGA87160.16076526924216958No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT83540.15408823534294225No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA82900.1529077652613109No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC80300.14811210555468354No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT79240.1461569519819816No Hit
TTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGG78420.14464447468989144No Hit
CATGTAGACTGTGGTCGTGGACGTGTCCCTGGTCATGGTGACTCTGCCCT78070.1439989051139993No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG78040.14394357057892282No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT77830.14355622883338753No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTT77590.14311355255277577No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAGGGTTTCCTGCA77410.14278154534231696No Hit
ACTATATACACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATG73820.1361598459781661No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC72910.13448136508084654No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA71190.13130885173646228No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAG69890.1289110218831486No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT69550.12828389715228194No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCTACTCATAT68290.1259598466790702No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC66420.1225106606593036No Hit
GTTGTATAACTCCCCGAACCATAATCTCTCGCACAGTAATACACGGCCGT65980.12169908747818206No Hit
CTCCCAGGTGCAGCTGGTGCAGTCTGGGGCTGAGGTGAAGAAGCCTGGGG65650.1210904075923409No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCT65540.1208875142970605No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTG65450.1207215106918311No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC65120.12011283080598993No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT65000.11989149266568404No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT64210.11843434990867034No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACAC64180.11837901537359388No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG62910.11603652005535665No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGTAATTATAACTATGTCTCCT61430.11330668299158417No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT60870.11227377167015674No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGG60550.11168353662934105No Hit
GTATTACTGTGCGAGAGATTATGGTTCGGGGAGTTATACAACCGACTACT59510.10976527274669011No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC58070.10710921506301958No Hit
ATACACGGCCGTGTCTTCAGATCTCAGGCTGCTCAGGTCCATGTAGACTG57430.10592874498138823No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC56960.10506183726519021No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT56930.10500650273011375No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG56470.1041580398589412No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT56420.10406581563381376No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGT56290.10382603264848238No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG55420.10222133113126476No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT55350.10209221721608633No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG55350.10209221721608633No Hit
CAGTAATACACGGCCGTGTCTTCAGATCTCAGGCTGCTCAGGTCCATGTA54550.10061662961404715No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)