FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005106819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5066732
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGCATGGGATGACAGCCTGAGTGTCGTGTTCGGCGG114010.22501683530922892No Hit
TTACTGTACTAGGCAGGCGGTTCCAGCAGCTGTTTGGTTCGACCCCTGGG111490.22004321523222464No Hit
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG108890.21491170245436308No Hit
CTCCTGTACAGCTTCTGGATTCACCTTTGGTGATTATGCTATGAGCTGGT106990.2111617508090027No Hit
GTACTAGGCAGGCGGTTCCAGCAGCTGTTTGGTTCGACCCCTGGGGCCAG89260.1761687809815084No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCC86630.17097805844082536No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA79390.1566887690132417No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT78330.15459669072688273No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC77990.1539256467482393No Hit
GTAATACACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGATAGG77540.1530375003059171No Hit
GTACAGTAATACACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAG77330.15262303196616675No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA77300.15256382220334527No Hit
TTTAAAAGGTGTCCAATGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCT75860.14972175358791426No Hit
GTGTATTACTGTACTAGGCAGGCGGTTCCAGCAGCTGTTTGGTTCGACCC73550.14516260185066035No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT69870.13789953761122553No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA69050.13628113742743841No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC68910.13600482520093818No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG68750.13568903979922364No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT67510.13324170293593582No Hit
ATACACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGATAGGCGA63130.12459707756399983No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA62970.12428129216228527No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACGACACTCAGGCTG62780.12390629699774924No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG62110.12258394562806954No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC62030.12242605292721224No Hit
GTATTACTGTACTAGGCAGGCGGTTCCAGCAGCTGTTTGGTTCGACCCCT61110.12061028686735356No Hit
CCCTGAGACTCTCCTGTACAGCTTCTGGATTCACCTTTGGTGATTATGCT59290.11701822792285048No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT57830.11413668613220515No Hit
GCCTAGTACAGTAATACACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATT57510.11350511532877602No Hit
GCCGTGTATTACTGTACTAGGCAGGCGGTTCCAGCAGCTGTTTGGTTCGA57410.11330774945270443No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT57350.11318932992706147No Hit
ACAGAATACGCCGCGTCTGTGAAAGGCAGATTCACCATCTCAAGAGATGA55440.10941964169409395No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC55110.10876833430305767No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC54110.10679467554234169No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT53020.10464338749316127No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA52220.10306446048458848No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC52210.10304472389698133No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA51320.1012881675999441No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)