FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005106820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5066732
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGCATGGGATGACAGCCTGAGTGTCGTGTTCGGCGG115930.2288062601298036No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT103290.20385921339435362No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA93310.18416209896240812No Hit
CTCCTGTACAGCTTCTGGATTCACCTTTGGTGATTATGCTATGAGCTGGT92540.1826423817166568No Hit
TTACTGTACTAGGCAGGCGGTTCCAGCAGCTGTTTGGTTCGACCCCTGGG90840.17928716182343965No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCC83640.1650768187462846No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC81910.16166238909024594No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG81210.16028082795774476No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT75840.14968228041269993No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC75150.14832045586780593No Hit
GTAATACACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGATAGG74600.14723494354941213No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA72400.14289289427583698No Hit
GTACAGTAATACACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAG71840.141787645369836No Hit
GTACTAGGCAGGCGGTTCCAGCAGCTGTTTGGTTCGACCCCTGGGGCCAG70860.13985345978433436No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT70570.13928109874372674No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC69340.13685349846804606No Hit
TTTAAAAGGTGTCCAATGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCT68870.13592587885050955No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT67030.13229434673079216No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA66250.1307548928974337No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA65550.12937333176493251No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT62660.12366945794646332No Hit
GTGTATTACTGTACTAGGCAGGCGGTTCCAGCAGCTGTTTGGTTCGACCC59610.11764979872627959No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC58640.11573534972838509No Hit
ATACACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGATAGGCGA58230.11492614963649153No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT58000.11447220812152686No Hit
GGAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG55500.10953806121973689No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA55230.10900517335434359No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC54550.10766308539705673No Hit
GCCTAGTACAGTAATACACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATT54450.10746571952098512No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC53670.10592626568762666No Hit
CCCTGAGACTCTCCTGTACAGCTTCTGGATTCACCTTTGGTGATTATGCT52520.10365655811280328No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT52180.10298551413415985No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC50950.1005579138584792No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)