FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106832

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106832
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3381005
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAACATGGGATGCGTCCCTGAAGGGTCTGGTTTTCGG103390.3057966492211635No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGTCCACCGATGGCCAGGGAGG100550.29739677995152325No Hit
GTGTACACCTGTGGCTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT99370.2939066934239967No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG79860.23620195770192592No Hit
GGTGTACACCCTGCCCCCATCCCAGGAGGAGATGACCAAGAACCAGGTCA66880.19781100589913356No Hit
GTCCTGGGCCCAGTCTGTGTTGACTCAGCCACCCTCAGCGTCTGGGACCC53810.1591538610561061No Hit
TCCCTGAAGGGTCTGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTACG53810.1591538610561061No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC49130.14531182296388204No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA43760.12942897156318905No Hit
GTTCCTGATGGGTCCGTCAGTGATGACTCCTTCAACTGGATCCGCCAGTC43280.12800927534860196No Hit
TATTTGGACTCAACTCTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATC43220.1278318133217786No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT42260.12499242089260441No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT42120.12457834283001652No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCC41650.12318822361989999No Hit
GTGTTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGGGGGTCAC41080.12150233436507783No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA40610.12011221515496132No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGA39990.11827844087778634No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGAACTGCT39780.11765732378390449No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA39230.11603058853802345No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT38430.11366442818037832No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC37820.1118602309076739No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT37610.11123911381379205No Hit
GTGGTGGACGTGAGCCAGGAAGACCCCGAGGTCCAGTTCAACTGGTACGT36600.10825183636226507No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT35670.10550117494650259No Hit
ATTCTAGGTTCAGCCCGCTGGACGTCTGGGGCAATGGGACTACGGTCATT34340.10156743335191755No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC34270.1013603943206236No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA34170.10106462427591795No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG34080.10079843123568287No Hit
CAATAGATGAGTCCAACAATCGCGTCTTCCTGAGGCTGATGTCTGTGACC33930.10035477616862443No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)