FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005106834

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106834
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3189211
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAATAGTTACCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCA141190.442711379084043No Hit
GTAATACACGGCCGTGTCGTCAGATCTCAGGCTCCTCAGCTCCATGTAGG126340.3961481382072243No Hit
CTATTATAGTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCT106420.33368754842498666No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT91400.286591260346211No Hit
GTGTATTACTGTGCGAGAGGGTCTGGTTACGGTGACTACCCCTATGAATA87190.2733905031683385No Hit
GCCTACATGGAGCTGAGGAGCCTGAGATCTGACGACACGGCCGTGTATTA79320.24871355328951267No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT79010.24774152603888547No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA73510.23049588126969334No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG71530.2242874491527842No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC66480.2084528116828896No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC63400.19879525061214198No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA63160.19804271338584997No Hit
CCATGTAGGCTGTGCTCGTGGATGTGTCTGTGGCCATGGTGACTCTGCCC60570.1899215824854486No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC58880.1846224661836423No Hit
CACTATAATAGTTACCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAAT58630.1838385732395881No Hit
ATTATAGTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGG57510.18032673285022532No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA55830.1750589722661812No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT55580.17427507932212702No Hit
GTGCTGGAAGTATTCATAGGGGTAGTCACCGTAACCAGACCCTCTCGCAC54400.17057510462619124No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT51470.16138787932187618No Hit
GTCTGGAGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA50920.15966331484495697No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT50480.15828366326342158No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC50260.1575938374726539No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAAGTTCAGCG49130.15405064136552896No Hit
CATGTAGGCTGTGCTCGTGGATGTGTCTGTGGCCATGGTGACTCTGCCCT48670.15260827834846927No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC43880.13758888954039103No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC42670.13379484769116876No Hit
CTTCCAGCACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAGCCTCCA40390.12664574404139456No Hit
GTATTACTGTGCGAGAGGGTCTGGTTACGGTGACTACCCCTATGAATACT39930.12520338102433487No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGCATTAGCAA39560.12404321946713466No Hit
CTCCCAGGTTCAGCTGGTGCAGTCTGGAGCTGAGGTGAAGAAGCCTGGGG39440.12366695085398866No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC39190.12288305790993446No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA38890.12194238637706943No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC38870.1218796749415451No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT38600.12103307056196659No Hit
ATACACGGCCGTGTCGTCAGATCTCAGGCTCCTCAGCTCCATGTAGGCTG37870.11874410316532835No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT37760.1183991902699445No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG36850.11554581995358727No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG36850.11554581995358727No Hit
CAGTAATACACGGCCGTGTCGTCAGATCTCAGGCTCCTCAGCTCCATGTA35930.11266109391946785No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT35660.11181448953988934No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG34970.10965094501429978No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT34390.10783231338409406No Hit
CCTTGGGAGAATCCCCTAGATCACAGCTCCTCACCATGGACTGGACCTGG34360.10773824623080755No Hit
GTCCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAAGT33950.10645266180255869No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAGCGCTTACAATGGT33770.10588825888283968No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG33610.10538656739864499No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCA33260.10428911727696913No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32900.1031603114375311No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32560.10209421703361742No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA32160.1008399883231307No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32030.10043236399222254No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)