FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106867

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106867
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6218670
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATAAA269090.43271310424897924No Hit
ACTACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGA229960.3697896817165085No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT193270.3107899277498243No Hit
GTATATTATTGTGCGAAAGTTCTTAAAAGTGGGAGCTACTATTACGACCA190030.3055798104739438No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC187920.30218680200107095No Hit
ATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATAAAGTG168780.271408516612073No Hit
GTGTGAGGTGCAGTTGTTGGAGTCGGGGGGAGGCTTGGTACAGCCTGGGG135680.21818170123193542No Hit
ATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATT135650.21813345940530693No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA133220.21422587144839653No Hit
GCCGTATATTATTGTGCGAAAGTTCTTAAAAGTGGGAGCTACTATTACGA127100.20438453881617774No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT124310.19989804893972507No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA122650.1972286678662801No Hit
ATATTATTGTGCGAAAGTTCTTAAAAGTGGGAGCTACTATTACGACCAAG122370.1967784108177472No Hit
GTATGTAGTACCACCACTACTACTAATGGTTGAGACCCACTCCAGTCCCT121300.19505778566799653No Hit
GTACTACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGA119410.19201855059039957No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC117550.1890275573394311No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT108650.1747158154396358No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC107490.17285046480999955No Hit
GTACGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAG107310.17256101385022843No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG103210.16596796421099688No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC102220.1643759839322556No Hit
CAATAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA98510.15841007803919488No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT97540.15685025897820595No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG90170.14499885023646536No Hit
ATGTAGTACCACCACTACTACTAATGGTTGAGACCCACTCCAGTCCCTTC89580.14405009431277108No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC89000.14311741899795294No Hit
GAATACACCTTTAGCAATTATGCCATGAACTGGGTCCGCCAAGCTCCAGG88120.14170232541684957No Hit
CCTTTAGCAATTATGCCATGAACTGGGTCCGCCAAGCTCCAGGGAAGGGA86310.138791735210262No Hit
GTCTGCATCTGTACGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA84210.13541480734626535No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG84140.13530224308413213No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTGTTTCTTGT83570.1343856483781902No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT82800.13314744149472477No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG81360.13083183381655564No Hit
TAATAGTAGCTCCCACTTTTAAGAACTTTCGCACAATAATATACGGCCGT80030.12869311283602441No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTACGAGACAGAGTCACCATCACTT79530.1278890823922157No Hit
GATCTATGCTGCTTCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTG79360.12761571204132074No Hit
GAGTTACAGTAACTCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCA78880.12684384281526434No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA77990.12541266862528483No Hit
GCCTGGGGGGTCCCTGAGAGTCTCCTGTGCAGCCTCTGAATACACCTTTA77010.12383676895541973No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT76720.12337043129801066No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA76520.1230488191204872No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA75270.12103874301096537No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC74770.12023471256715663No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTGTTT74460.11973621369199523No Hit
CTACTATTACGACCAAGGGCATGATGCTTTTGATATCTGGGGCCAAGGGA73990.11898042507481503No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG73710.11853016802628215No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG73520.11822463645763484No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG72860.11716331627180732No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG69480.11172807047166035No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA68590.1102968962816808No Hit
GCGTAGTATGTAGTACCACCACTACTACTAATGGTTGAGACCCACTCCAG67670.10881748026507275No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC67410.10839938443429223No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT67280.10819033651890196No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT66620.10712901633307445No Hit
CTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATTCCA66040.10619634101825633No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG65960.10606769614724691No Hit
TAGTAGTAGTGGTGGTACTACATACTACGCAGACTCCGTGAAGGGCCGGT65800.10581040640522812No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC65590.10547271361882847No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG63710.10244955915010767No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC63620.1023048336702221No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGC62980.10127567470214693No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)