FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106880

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106880
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences775164
Sequences flagged as poor quality0
Sequence length101
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG133631.7238932664571625No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118511.5288377685238221No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70640.9112910300271942No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67880.8756856613568226No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA67390.8693644183682421No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA50610.6528940972490982No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA48990.6319952938990975No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA48890.6307052443095913No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG37470.4833815811879809No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35790.46170874808427637No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA35620.4595156637821157No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA35080.4525493959987822No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAA34080.43964890010372No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG33220.4285544736339665No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30930.39901233803427405No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA29100.37540443054631023No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT26070.33631592798427173No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA25910.3342518486410618No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA24330.31386906512686347No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA23190.29916249980649257No Hit
GTATCAACGCAGAGTACGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA22670.29245424194106023No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22140.2856169791166772No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG20900.26962036420680013No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT20820.26858832453519516No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT20250.2612350418750097No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG20030.25839693277809594No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT19510.25168867491266367No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA18900.2438193724166757No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA18170.23440201041328027No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG17890.23078987156266287No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA17840.23014484676790975No Hit
GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17560.22653270791729235No Hit
TATCAACGCAGAGTACGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAA17140.2211144996413662No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT16680.2151802715296376No Hit
GTATCAACGCAGAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAA16070.20731096903364965No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA15810.20395684010093348No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG15550.20060271116821732No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAA15130.1951845028922912No Hit
GAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA14970.19312042354908124No Hit
GGTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA14390.18563813592994516No Hit
GAGTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA14230.1835740565867352No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT13760.17751082351605596No Hit
GAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13750.17738181855710536No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT13540.17467271441914228No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG13510.17428569954229042No Hit
GTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13260.17106057556852486No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT13170.16989953093796925No Hit
GTACGGGAAGCAGCGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA12820.16538435737469748No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAA12620.16280425819568506Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAA11950.15416092594599334No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA11950.15416092594599334No Hit
GAGTACGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11660.15041978213642532No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAA11450.14771067799846227No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT11050.14255047964043738No Hit
GTATCAACGCAGAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA10840.1398413755024743No Hit
GAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA10730.13842232095401746No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT10640.13726127632346188No Hit
GGTATCAACGCAGAGTACGGGGGTATCAACGCAAAAAAAAAAAAAAAAAA10050.12964998374537517No Hit
GTATCAACGCAGAGTACGGGAAGCAGCGGTATCAACGCAAAAAAAAAAAA9820.12668286968951087No Hit
GGTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA9790.12629585481265898No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATAC9460.12203869116728847Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA9450.12190968620833785No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT8800.11352436387654741No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT8720.11249232420494244No Hit
GTACGGGAAGTAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA8670.11184729941018934No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT8650.11158928949228808No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT8580.11068625477963373No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT8570.1105572498206831No Hit
GTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8350.10771914072376942No Hit
ATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA8340.1075901357648188No Hit
TATCAACGCAGAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA7980.10294595724259639No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT7850.10126889277623832No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)