FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4522144
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTATGGTGGGTCCTTCAGTGGTTACTACTGGAGCTGGATCCGCCAGCC198360.43864149394623436No Hit
GTAATACACAGCCGTGTCCGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA159990.3537923604378808No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG150810.3334922550011676No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG149020.32953395557505466No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT124380.2750465266033103No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC110780.24497229632669815No Hit
GTGTATTACTGTGCGAGAGGCCTGGATTACTATGGTTCGGGGAGCAGCAT110710.24481750249439205No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT98710.21828141695620484No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG70170.15516976018454962No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT68670.15185274949227623No Hit
GTAGTAGCCCATGCTGCTCCCCGAACCATAGTAATCCAGGCCTCTCGCAC66530.14712048090463284No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT64810.14331697531082602No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAG63680.1408181605893134No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT62100.13732424266011872No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG60130.13296790195093303No Hit
CCGTAGTAGTAGCCCATGCTGCTCCCCGAACCATAGTAATCCAGGCCTCT56450.1248301690525556No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG56280.12445424117409797No Hit
ATACACAGCCGTGTCCGCGGCGGTCACAGAGCTCAGCTTCAGGGAGAACT56270.12443212776948279No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG55690.12314955030180373No Hit
GTCCTGATCTATGCTGCATCCAGTCTGCAAAGTGGGGTCCCATCAAGGTT55560.12286207604180673No Hit
GATCTATGCTGCATCCAGTCTGCAAAGTGGGGTCCCATCAAGGTTCAGTG53880.11914702406646051No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC53510.11832882809569975No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC53180.1175990857433996No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC53060.11733372488801772No Hit
GGCTACTACTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGT51480.11383980695882306No Hit
CCCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATACCGT51280.11339753886651996No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG51160.11313217801113809No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT50430.11151789947423169No Hit
ACCATAGACAGCGCAGGTGAGGGACAGGGTCTCCGAAGGCTTCAACAGTC50120.11083238393116185No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA49950.11045645605270421No Hit
TAGTAATCCAGGCCTCTCGCACAGTAATACACAGCCGTGTCCGCGGCGGT49490.10943923944040702No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACT47990.10612222874813364No Hit
CAGTAATACACAGCCGTGTCCGCGGCGGTCACAGAGCTCAGCTTCAGGGA46060.10185434165740852No Hit
GTATTACTGTGCGAGAGGCCTGGATTACTATGGTTCGGGGAGCAGCATGG45600.10083712504511134No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT45400.10039485695280823No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)