FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106893

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106893
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3983154
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTAATTTGCACATACA132680.33310286270628753No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT102060.2562291088920991No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC94550.23737470356406004No Hit
GCTTAATAGTTACCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCA66840.1678067179928268No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAGCAA58060.14576388459999287No Hit
ATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTAATTTGCACATACAGCG55280.13878449088335526No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA54180.1360228602760526No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT52160.1309515022517332No Hit
CTATTAAGCTGTTGACAGTAATAAGTTGCAAAGTCTTCAGGCTGCAGGCT51550.1294200525513199No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA51160.12844092897236714No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG46720.11729398361198186No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC46720.11729398361198186No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC45610.11450724727188553No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG45210.11350301796013912No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT44780.11242347145001173No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG44620.11202177972531316No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG43630.10953631217874077No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT42550.10682489303702543No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC42200.10594619238924732No Hit
ATAGTTACCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA40770.10235607259975385No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC40430.1015024776847694No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)