FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005106905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4209046
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTATTATAGTGGGAGTACCTACTACAACCCGTCCCAACCGTCCCTCAA222040.5275304665237681No Hit
GTAGTACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTCCAGGGAGA131240.31180462271022935No Hit
ATAATAGATAGTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGA125300.29769216112154634No Hit
CTATTATAGTGGGAGTACCTACTACAACCCGTCCCAACCGTCCCTCAAGA117750.2797546047251563No Hit
ACCTACTACAACCCGTCCCAACCGTCCCTCAAGAGTCGAGTCACCATGTC93890.22306717484199506No Hit
GTCTCTGGTGGCTCCATCAGCACTAGTAGTTACTACTGGGGCTGGATCCG93840.2229483830777806No Hit
GTGTACTACTGTGCGAGACTAGAGGACCTCTGGGGGTCCTCTGACTACTG93290.221641673671421No Hit
ACTACAACCCGTCCCAACCGTCCCTCAAGAGTCGAGTCACCATGTCCGTA74760.17761744585352596No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC74450.17688093691539603No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA66740.15856324687351955No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC62270.14794326315274292No Hit
ATTATAGTGGGAGTACCTACTACAACCCGTCCCAACCGTCCCTCAAGAGT60930.1447596438717942No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT60230.14309655917279115No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG59000.14017428177311436No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC59000.14017428177311436No Hit
CTATAATAGATAGTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCG58300.13851119707411133No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT58260.13841616366273973No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG57450.13649173708246476No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA54010.1283188637045069No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT50210.11929068962420464No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACAATGCCAGTG49920.1186016973917605No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT49640.11793646351215929No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA49500.11760384657235867No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA49250.11700988775128615No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT48240.11461029411415319No Hit
GTACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTCCAGGGAGAACT47350.11249580071113502No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA45390.10783916355392648No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA44180.10496440285993548No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC43070.1023272256943735No Hit
GTACTTGATGAAGCTTTACAGTGATGGCGGCCACATCAAGGGGGACGGGA42810.10170950852045808No Hit
GTAATAGTCAGCCTCATCCTCAGACTGGAGGCTGGAGATGGTGAGGTAGC42540.10106803299369975No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)