FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2846122
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGT118510.41639114556579093No Hit
CCCCTATATTTGTCATCTATGGTAAAAACAACCGGCCCTCAGGGATCCCA97920.3440470928512551No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCC94620.33245236852109644No Hit
ATATAGGGGCCTGTCCTGGCTTCTGCTGGTACCAGCTTGCATAATAGCTT90430.3177305821746222No Hit
GTGTATTACTGTGCGAGGCACGACTACAGTAACTACCAGAATTTTGACTA89670.31506028202585834No Hit
GTAATACACAGCCGTGTCCGCAGCGGTCACAGAGCTCAGCTTCAGGGAGA86490.30388718403497816No Hit
CTATTATTGTAACTCCCGGGGCGGCAGTGGTAGCCATCCTTGGGTGTTCG85700.30111147730139465No Hit
GTCTCTGGTGGCCCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCC79860.28059232878984103No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT77860.2735652231351994No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCAC70720.2484784559481287No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA69680.24482436100771504No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA68610.2410648594824818No Hit
CTATTATGCAAGCTGGTACCAGCAGAAGCCAGGACAGGCCCCTATATTTG68130.2393783541253678No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT67860.23842969486199114No Hit
CAATAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGA64560.22683497053183244No Hit
AGCCAGGACAGGCCCCTATATTTGTCATCTATGGTAAAAACAACCGGCCC60970.2142213158817507No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC60900.21397536718383822No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC57760.20294281130605082No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCT56600.1988670900263587No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT55670.1955994858969503No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG55490.19496704638803256No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG52950.18604262220663767No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA52230.18351286417096666No Hit
CTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGTCA51840.18214257856831156No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA51060.1794020073630013No Hit
GTATTACTGTGCGAGGCACGACTACAGTAACTACCAGAATTTTGACTACT49890.17529115055503594No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT49320.17328842544346307No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG48770.1713559713884366No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA48480.17033704106851358No Hit
GGGTATATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAG47500.16689375929773917No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC46300.16267749590495417No Hit
GTCCTGGCTTCTGCTGGTACCAGCTTGCATAATAGCTTCTGAGGCTGTCT46090.1619396498112168No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT45970.1615180234719383No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGA45650.16039368656719566No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC45050.15828555487080315No Hit
CTATATTTGTCATCTATGGTAAAAACAACCGGCCCTCAGGGATCCCAGAC44570.15659904951368916No Hit
GTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCC43100.15143412685752755No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA42960.1509422294617026No Hit
GTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTG42730.15013411231141882No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA42530.14943140174595468No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC42510.14936113068940826No Hit
GACTATTATTGTAACTCCCGGGGCGGCAGTGGTAGCCATCCTTGGGTGTT41950.1473935411061086No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGGATGGCTA41590.14612866208827308No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTG41430.14556649363590177No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG41170.14465296990079834No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT41120.14447729225943232No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG41010.144090801448427No Hit
ATAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAG40560.14250970267613264No Hit
TTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGT40490.1422637539782202No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG40310.14163131446930244No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC40310.14163131446930244No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA40260.1414556368279364No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT39560.13899614984881184No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC39140.13752045766133708No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG39110.13741505107651744No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC37040.13014199672396334No Hit
GCATAATAGCTTCTGAGGCTGTCTCCTTGGCATGTGATCCTGACTGTCTG36980.1299311835543241No Hit
ATATTTGTCATCTATGGTAAAAACAACCGGCCCTCAGGGATCCCAGACCG36020.1265581728400961No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC35740.1255743780484463No Hit
GTCAGGATCACATGCCAAGGAGACAGCCTCAGAAGCTATTATGCAAGCTG35270.12392300821960549No Hit
GTGGTAGCCATCCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTA34940.1227635357865896No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA34860.12248245156040395No Hit
CAGTAATACACAGCCGTGTCCGCAGCGGTCACAGAGCTCAGCTTCAGGGA34430.12097162384465598No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC34280.12044459092055786No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT33890.11907430531790275No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACA33750.11858240792207785No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT33630.11816078158279934No Hit
GCCCTCAGGGATCCCAGACCGATTCTCTGGCTCCAGCTCAGGGAACACAG33550.11787969735661367No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGG32590.11450668664238568No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT32330.11359316290728226No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT32280.11341748526591622No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCT31980.11236341941771998No Hit
CACCTGCACTGTCTCTGGTGGCCCCATCAGTAGTTACTACTGGAGCTGGA31730.11148503121088976No Hit
ATCCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC31690.11134448909779693No Hit
CTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACA31320.11004447455168823No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA31300.10997420349514182No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA30880.10849851130766705No Hit
CAAATATAGGGGCCTGTCCTGGCTTCTGCTGGTACCAGCTTGCATAATAG30800.1082174270814814No Hit
ATACACAGCCGTGTCCGCAGCGGTCACAGAGCTCAGCTTCAGGGAGAACT30750.10804174944011537No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG30680.10779580074220291No Hit
GCTGTGTATTACTGTGCGAGGCACGACTACAGTAACTACCAGAATTTTGA30150.10593361774372287No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT29920.10512550059343906No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGA29750.10452819661279453No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA29740.10449306108452133No Hit
GTAATAGATATACCCAATCCACTCCAGTCCCTTCCCTGGGGGCTGCCGGA29560.10386062157560358No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC29220.10266601361431449No Hit
TCCTTGACCATCACTGGGGCTCAGGCGGAAGATGAGGCTGACTATTATTG28830.10129572801165937No Hit
CTTTCTGAGAGTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGT28650.10066328850274163No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGG28600.10048761086137559No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)