FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106908

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106908
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2846122
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGT131180.4609078598879458No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCC92170.3238441640941604No Hit
GTAATACACAGCCGTGTCCGCAGCGGTCACAGAGCTCAGCTTCAGGGAGA91240.32057655996475204No Hit
ATATAGGGGCCTGTCCTGGCTTCTGCTGGTACCAGCTTGCATAATAGCTT86070.3024114918475034No Hit
CCCCTATATTTGTCATCTATGGTAAAAACAACCGGCCCTCAGGGATCCCA78070.27430306922893677No Hit
CTATTATTGTAACTCCCGGGGCGGCAGTGGTAGCCATCCTTGGGTGTTCG74530.26186509222022103No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG74350.2612326527113033No Hit
GTGTATTACTGTGCGAGGCACGACTACAGTAACTACCAGAATTTTGACTA73670.2588434367887252No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT70080.24622978213864338No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT66180.2325269261120922No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT64800.22767822321038944No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA64600.22697551264492527No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCAC64530.22672956394701282No Hit
GTAATAGATATACCCAATCCACTCCAGTCCCTTCCCTGGGGGCTGCCGGA63970.22476197436371317No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA63280.2223376229128618No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGGATGGCTA62060.21805108846353038No Hit
CAATAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGA60690.21323752109010086No Hit
CTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGTCA59780.2100401880172389No Hit
GGGTATATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAG57640.20252118496677232No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC56990.2002373756290138No Hit
CTATTATGCAAGCTGGTACCAGCAGAAGCCAGGACAGGCCCCTATATTTG55530.1951075885011254No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT54690.1921562041261759No Hit
GTCTCTGGTGGCCCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCC54600.191839984371717No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCT53610.1883615670726694No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA53140.18671019724382862No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG53090.1865345196024626No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC53050.18639397748936973No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA51810.18203717198349192No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG50660.17799658623207296No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC50120.17609926770531972No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG49440.17371005178274157No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA47640.1673856566935641No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC47630.16735052116529087No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA47620.16731538563701767No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA46640.16387210386624326No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT46490.16334507094214512No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC45210.15884772332317448No Hit
GTCCTGGCTTCTGCTGGTACCAGCTTGCATAATAGCTTCTGAGGCTGTCT44800.15740716666397292No Hit
AGCCAGGACAGGCCCCTATATTTGTCATCTATGGTAAAAACAACCGGCCC44740.1571963534943337No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGA43140.15157466897062039No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA42940.15087195840515621No Hit
GTATTACTGTGCGAGGCACGACTACAGTAACTACCAGAATTTTGACTACT41620.14623406867309272No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA41530.14591784891863385No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC41110.1444421567311591No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA41050.14423134356151984No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT40820.14342322641123606No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGGATGGCTAC40430.14205294080858094No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA39570.13903128537708503No Hit
ATAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAG39510.13882047220744578No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG39160.1375907287178835No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG38660.13583395230422307No Hit
TTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGT38060.1337258206078306No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT37800.13281229687272716No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT37660.13232039947690227No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC37410.13144201127007205No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACA37190.13066902964806146No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC37040.13014199672396334No Hit
GTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTG37040.13014199672396334No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC36820.12936901510195278No Hit
CTATATTTGTCATCTATGGTAAAAACAACCGGCCCTCAGGGATCCCAGAC36470.12813927161239047No Hit
GATATACCCAATCCACTCCAGTCCCTTCCCTGGGGGCTGCCGGATCCAGC36400.127893322914478No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC35850.12596086885945157No Hit
GACTATTATTGTAACTCCCGGGGCGGCAGTGGTAGCCATCCTTGGGTGTT35560.12494193853952852No Hit
CAGTAATACACAGCCGTGTCCGCAGCGGTCACAGAGCTCAGCTTCAGGGA35450.12455544772852323No Hit
GTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCC35280.1239581437478787No Hit
GCATAATAGCTTCTGAGGCTGTCTCCTTGGCATGTGATCCTGACTGTCTG34770.12216623180594506No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT34040.11960133824200088No Hit
GTGGTAGCCATCCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTA33880.11903916978962953No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT33520.11777429077179404No Hit
ATACACAGCCGTGTCCGCAGCGGTCACAGAGCTCAGCTTCAGGGAGAACT33490.11766888418697442No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTG33370.11724725784769592No Hit
ATCCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC33200.11664995386705136No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC33130.11640400516913892No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA32540.11433100900101964No Hit
CTGTAATAGATATACCCAATCCACTCCAGTCCCTTCCCTGGGGGCTGCCG32050.11260936811563244No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGG31970.11232828388944675No Hit
GTATCAACGCAGAGTACGGGAGCTGTGGGCTCAGAAGCAGAGTTCTGGGG31730.11148503121088976No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGG31610.11106340487161126No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG31190.10958771268413652No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT30620.10758498757256366No Hit
CCCAAGGATGGCTACCACTGCCGCCCCGGGAGTTACAATAATAGTCAGCC30590.10747958098774402No Hit
GTACGGGAGCTGTGGGCTCAGAAGCAGAGTTCTGGGGTGTCTCCACCATG30590.10747958098774402No Hit
CTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACA30220.10617956644163533No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT30130.10586334668717644No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGA29350.1031227754818662No Hit
GTCAGGATCACATGCCAAGGAGACAGCCTCAGAAGCTATTATGCAAGCTG29300.10294709784050016No Hit
CAAATATAGGGGCCTGTCCTGGCTTCTGCTGGTACCAGCTTGCATAATAG29130.10234979385985563No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT29050.10206870963366996No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)