FastQCFastQC Report
Sun 7 Aug 2022
EGAF00005106927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4317418
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT140480.3253796597874007No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC134720.31203835255238205No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTAATTTGCACATACA120920.280074803968483No Hit
GCTTAATAGTTACCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCA96990.22464815776466396No Hit
CTATTAAGCTGTTGACAGTAATAAGTTGCAAAGTCTTCAGGCTGCAGGCT82990.19222136934621573No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAGCAA71900.16653472052045923No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT70270.16275931586888273No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG70010.16215710408396872No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC67730.15687616997010712No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG62240.1441602365117299No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA58160.134710143886925No Hit
ATAGTTACCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA57140.1323476207307238No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT55230.12792368031077833No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG53980.12502843134484545No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC53610.12417143765092932No Hit
ACTTATTACTGTCAACAGCTTAATAGTTACCCGCTCACTTTCGGCGGAGG52790.12227215432927736No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC50540.11706070619059818No Hit
TATTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAACTCCTGAT50000.11580995863731518No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT49550.11476766900957933No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC49410.11444340112539485No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG49190.11393383730739068No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA48440.11219668792783093No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA48250.11175661008500914No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC47830.11078380643245568No Hit
ATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTAATTTGCACATACAGCG47480.10997313672199448No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA46600.10793488144997775No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT46000.10654516194632997No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA45790.10605876012005323No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA44310.10263078534438871No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT44080.10209805953465706No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC43670.10114841787383108No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC43190.10003664227091284No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)