FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005106928

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106928
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4317418
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT142060.3290392544803399No Hit
CTATTAAGCTGTTGACAGTAATAAGTTGCAAAGTCTTCAGGCTGCAGGCT118450.27435379201179966No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTAATTTGCACATACA117250.2715743530045041No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC108090.250357968582148No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG105630.24466011861719203No Hit
GCTTAATAGTTACCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCA95130.22034002730335583No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT71760.16621045263627474No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG70780.16394057744698334No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT63920.14805145112194373No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG62900.14568892796574248No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC62300.1442992084620947No Hit
ATAGTTACCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA61920.14341905277645112No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC58330.1351038977462919No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAGCAA58290.13501124977938203No Hit
ACTTATTACTGTCAACAGCTTAATAGTTACCCGCTCACTTTCGGCGGAGG58060.13447852396965038No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC56820.13160643699544497No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA51700.11974749723098388No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA50280.11645849440568413No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG48750.1129147096713823No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC48530.1124051458533781No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA48510.11235882186992317No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA48310.11189558203537392No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA47040.10895400908598613No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT46970.10879187514389387No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA45040.1043216107404935No Hit
ATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTAATTTGCACATACAGCG44800.1037657229390344No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG44410.10286240526166333No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT44000.10191276360083734No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA43970.10184327762565495No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG43450.1006388540558269No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC43260.10019877621300509No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)