FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4035412
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTATGGTGGGTCCTTCAGTGGTTATTATTGGAGCTGGATCCGGCAGCC139290.34516921692258434No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG127080.31491208332631215No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG119850.2969956970936301No Hit
GTAATACACAGCCGTGTCCGCGGCGGTCACAGAGGTCAGGTTCAGGGAGA109640.2716946869365507No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC79260.19641117189521168No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT78870.1954447278245691No Hit
GTGTATTACTGTGCGAGAGGGGGTAACGGTGGCTACCATGACTACTGGGG78780.19522170226980542No Hit
GATTATTACTGCAGCTCATATACAAGCAGCAACACTTATGTCTTCGGAAC78470.19445350313673054No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT71840.1780239539358063No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC69680.17267134062147804No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC67670.16769043656508928No Hit
GGTTATAACTATGTCTCCTGGTACCAACAGCTCCCAGGCAAAGCCCCCAA67530.16734350792434577No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT63040.15621701080335787No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA62770.15554793413906684No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTGTTGCTGC61200.15165737723930048No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC61180.15160781600490855No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG60870.15083961687183367No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT60670.15034400452791438No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG57930.1435541154162202No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA54380.13475699631165294No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA52700.13059385262273096No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTGTTGCTG52390.12982565348965608No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT52100.12910701559097312No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT51920.12866096448144576No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT50630.1254642648631664No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG50460.125042994370835No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA49910.12368006042505697No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC49890.12363049919066504No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCT48100.11919476871258747No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC45950.11386693601545517No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG45740.11334654305433993No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT45150.11188448663977804No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC44410.1100507209672767No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT44300.10977813417812109No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC42610.10559020987200315No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG42100.10432639839500898No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACT42060.10422727592622513No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC41470.10276521951166324No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG41160.10199702037858836No Hit
TATACAAGCAGCAACACTTATGTCTTCGGAACTGGGACCAAGGTCACCGT40860.10125360186270943No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)