FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106934

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106934
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4035412
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTATGGTGGGTCCTTCAGTGGTTATTATTGGAGCTGGATCCGGCAGCC127090.3149368639435081No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG122730.30413251484606774No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG114100.2827468422059507No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG113210.28054136727550993No Hit
GTAATACACAGCCGTGTCCGCGGCGGTCACAGAGGTCAGGTTCAGGGAGA87230.2161613238003951No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT79410.19678288115315118No Hit
GTGTATTACTGTGCGAGAGGGGGTAACGGTGGCTACCATGACTACTGGGG68800.1704906463082332No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC67830.1680869264402247No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA67400.16702135990079825No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC62330.1544575869824444No Hit
GGTTATAACTATGTCTCCTGGTACCAACAGCTCCCAGGCAAAGCCCCCAA61300.15190518341126014No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT59900.14843589700382515No Hit
GATTATTACTGCAGCTCATATACAAGCAGCAACACTTATGTCTTCGGAAC59220.14675081503449958No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTGTTGCTGC58790.14568524849507314No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC58630.1452887586199377No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC57970.14365323788500406No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG57700.14298416122071303No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT57140.14159644665773904No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA53860.1334684042174628No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA52640.13044516891955518No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG51310.12714934683249196No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA51110.12665373448857267No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC50900.12613334152745742No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC50050.12402698906580047No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT49740.12325878993272557No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT49200.12192063660414353No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCT47780.11840178896231661No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT47500.11770793168082962No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTGTTGCTG47320.11726188057130227No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG46680.11567592107076055No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC45860.11364391046069151No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC45780.11344566552312378No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT45570.11292527256200853No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA45050.11163668046781841No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG43900.10878690949028254No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT43570.10796914912281572No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG42930.106383189622274No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT42660.10571411295798297No Hit
TTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGG42000.10407859222304934No Hit
ACCTACACCTGCGTGGTGGCCCATGAGGCCCTGCCCAACAGGGTCACCGA41970.10400425037146144No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)