FastQCFastQC Report
Sat 6 Aug 2022
EGAF00005106941

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106941
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2548809
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79720.31277353461950264No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT64580.25337324216918566No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48690.19103039890395868No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA47140.1849491272198113No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT44570.1748659864273863RNA PCR Primer, Index 27 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41060.16109484861360737No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA36080.14155631120260484No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT31460.12343019818275909RNA PCR Primer, Index 27 (96% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCGCGTATGCCGT29110.11421020562937434No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA27800.10907055020599817No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)