FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005106954

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106954
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4501729
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT166090.36894713120225586No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG141680.3147235206739455No Hit
ATATTACTGTCAACAGTTTGATAATCTCCCGCTCACCTTCGGCCAAGGGA140380.3118357413340519No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC120010.26658646044664175No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT85970.19097106911588857No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAA85460.18983817106716108No Hit
ACATATTACTGTCAACAGTTTGATAATCTCCCGCTCACCTTCGGCCAAGG84160.18695039172726746No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG83870.18630619479759888No Hit
CTATTATAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCA83730.18599520317637955No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT81580.18121926042194011No Hit
CAGTAATATGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGT75450.16760227014998014No Hit
ATCTATTATAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGT74630.16578074779712418No Hit
GTAATACACGGCCGTGTCCGCGGCGGTCACAGAGCTCAGTTTCAGGGAGA73390.16302625058061024No Hit
ATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATC72780.16167121565958323No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC70940.15758389720927227No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG68420.15198604802732463No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGTAGTTACTACTGGGGCTGGATCCG68220.15154177428272558No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG67700.15038666254676816No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT64920.14421125749684177No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC64250.14272294045243505No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC63960.14207874352276648No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA63420.1408792044123491No Hit
GTAATATGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAA59730.1326823538244972No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG57930.12868389012310602No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA57840.1284839669380365No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA57280.12724000045315922No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA56420.12532962335138345No Hit
ACTGTCAACAGTTTGATAATCTCCCGCTCACCTTCGGCCAAGGGACACGA56170.12477428117063467No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55800.12395237474312647No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCAATAGACACGTCC55100.12239741663702991No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA54950.12206421132858064No Hit
CTATAATAGATACTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCG54810.12175321970736132No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC54550.12117566383938261No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG54550.12117566383938261No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA54530.1211312364649227No Hit
ATTATAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACC54110.12019826160126476No Hit
AGTCAGGACATTAGCAACTATTTAAATTGGTATCAGCAGAAACCAGGGAA53540.11893208142915755No Hit
TTATCAAACTGTTGACAGTAATATGTTGCAAAATCTTCAGGCTGCAGGCT53180.11813238868887933No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC52930.11757704650813056No Hit
ATAATAGATACTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGA51630.11468926716823691No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT51480.11435606185978765No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC50010.11109064983698484No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG49300.10951347804365834No Hit
GTGTATTACTGTGGGAGAGATCGCTCCGCACGCAGTGGCTGGTACGGCGG48770.10833615262047093No Hit
GATCTACGATGCATCCAATTTGGAAGCAGGGGTCCCATCAAGGTTCAGTG48120.10689226295052412No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT47690.10593707439963623No Hit
GTTTGATAATCTCCCGCTCACCTTCGGCCAAGGGACACGACTGGAGATTA47450.10540394590611739No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG46750.10384898780002084No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC46470.1032270045575822No Hit
ATATTTTACTTTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACAT46440.10316036349589236No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC46190.10260502131514358No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT46000.10218296125777451No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)