FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106958

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106958
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3377832
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCAGTGTCCTCAGCCTTTAGGCTGCTGATCTGCAGAAATG115790.3427938393620523No Hit
GTATATTACTGTGCGAGAAGAGTCCTAGGATTTTGTACTAGTAACAGCTG92360.2734298212581325No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG83340.24672630255145903No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCC79450.2352100400493571No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT65470.19382254653280565No Hit
CTACTACTACATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTCCT62480.18497071494378645No Hit
TAGTAGTAGTGGCCAGCTGGGCAGCTGTTACTAGTACAAAATCCTAGGAC60520.17916817651085074No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT58860.1742537817156093No Hit
ATATACGGCAGTGTCCTCAGCCTTTAGGCTGCTGATCTGCAGAAATGCCG57680.17076041674067863No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT56630.1676519140087488No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA56440.16708942303820912No Hit
GGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAA54630.16173095642412055No Hit
GATTATTACTGCAGCTCATATGCAGGCAGCTGGGTGTTCGGCGGAGGGAC52860.15649090896172457No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTC50980.150925208832174No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA50100.14831998749493758No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA49630.14692856246254993No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA49350.14609962840070198No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG49060.1452410895509309No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC48620.14393847888231268No Hit
GCCGTATATTACTGTGCGAGAAGAGTCCTAGGATTTTGTACTAGTAACAG48360.14316875439631102No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGCTGCCTGCA48230.14278389215331017No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC46770.13846159311653156No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC45180.1337544318367521No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAATCAGCAGTGACGTT45060.1333991743816744No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA44890.13289589298698098No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG43610.12910648013281892No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT43210.12792228861589328No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA42360.12540588164242625No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG41610.12318552254819068No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT40310.11933690011818232No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC39310.11637642132586819No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT38890.11513302023309627No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG38720.11462973883840286No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA38540.11409685265578631No Hit
AGTAACAGCTGCCCAGCTGGCCACTACTACTACATGGACGTCTGGGGCAA37790.11187649356155072No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC36790.1089160147692366No Hit
CTCCCAGGTGCAGCTGGTGCAATCTGGGTCTGAGTTGAAGAAGCCTGGGG36680.10859036210208205No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT36400.10776142804023409No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT36320.10752458973684897No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT36200.10716933228177128No Hit
ATATTACTGTGCGAGAAGAGTCCTAGGATTTTGTACTAGTAACAGCTGCC35970.10648842215953902No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGG35490.10506739233922824No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA35340.10462332052038112No Hit
GTTATAACCACCAACGTCACTGCTGATTCCAGTGCAGGAGATGGTGACTG34470.10204770397106784No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC34350.10169244651599015No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA34140.10107074596960416No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCAGCTCATAT34070.10086351245414218No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA33920.10041944063529505No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA33880.1003010214836025No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)