FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3755060
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG112010.29829083956048635No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG107630.2866265785366945No Hit
GTAATACACGGCCGTGTCGTCAGATCTCAGGCTCCTCAGCTCCATGTAGG87970.2342705575942861No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC81040.21581545967308113No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG74330.19794623787635882No Hit
GATTATTACTGCTGCTCATATGCAGGTAGTAGCACTTTGGTATTCGGCGG69650.18548305486463598No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA64750.17243399572843043No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT63450.1689720004473963No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT61630.16412520705394854No Hit
GTTATAACCTTGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAA60260.1604767966423972No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA58790.15656207890153553No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAAAGTGCTACTA58350.15539032665257013No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT56970.15171528550808774No Hit
GCCTACATGGAGCTGAGGAGCCTGAGATCTGACGACACGGCCGTGTATTA56840.15136908597998433No Hit
GTGTATTACTGTGCGAGGTATACCTCCAATACTTGCTTTGACTACTGGGG54240.1444450954179161No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTT54050.1439391114922265No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG51200.13634935260688244No Hit
CCTTGGGAGAATCCCCTAGATCACAGCTCCTCACCATGGACTGGACCTGG50750.13515096962498602No Hit
GTCTGGAGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA50580.1346982471651585No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC50370.1341390017736068No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG49320.1313427748158485No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG49310.13131614408291745No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG47120.1254840135710215No Hit
TAGTAGCACTTTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTC46500.12383290812929754No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA45950.1223682178180908No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT45190.12034428211533238No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACT44540.11861328447481531No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT44270.11789425468567746No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATAACCTTGTCTCCT43690.11634967217567763No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG43580.11605673411343627No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT43320.11536433505722946No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT43070.11469856673395366No Hit
GTATTACTGTGCGAGGTATACCTCCAATACTTGCTTTGACTACTGGGGCC43040.11461867453516056No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA42670.11363333741671237No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA42070.11203549344085048No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT41740.11115667925412644No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAA41610.11081047972602302No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA41320.11003818847102309No Hit
ACCTACACCTGCGTGGTGGCCCATGAGGCCCTGCCCAACAGGGTCACCGA40960.10907948208550597No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC40870.10883980548912667No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA40360.10748163810964406No Hit
ATGCAACATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAG40190.10702891564981652No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC40130.10686913125223033No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG40030.10660282392292002No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39410.10495171848119604No Hit
CAGTAATACACGGCCGTGTCGTCAGATCTCAGGCTCCTCAGCTCCATGTA39190.10436584235671334No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG39040.10396638136274787No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC38970.10377996623223064No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAAAGTGCTACTAC38080.10140983100136883No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC38070.10138320026843778No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC37600.10013155582067929No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)