FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005106968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3755060
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG210460.5604704052664938No Hit
GTAATACACGGCCGTGTCGTCAGATCTCAGGCTCCTCAGCTCCATGTAGG110400.2940032915585903No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG105690.28146021634807433No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG105550.2810873860870399No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC78120.20803928565721985No Hit
GATTATTACTGCTGCTCATATGCAGGTAGTAGCACTTTGGTATTCGGCGG77730.2070006870729096No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG60710.16167517962429362No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT59880.15946482879101798No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT59130.1574675238211906No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA58300.15525717298791497No Hit
CCATGTAGGCTGTGCTCGTGGATGTGTCTGTGGTCATGGTGACTCTGCCC55630.1481467672953295No Hit
GTTATAACCTTGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAA55560.1479603521648123No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA55360.14742773750619165No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT54210.144365203219123No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTT52840.14071679280757166No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG52420.13959830202446832No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAAAGTGCTACTA51290.13658902920326174No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG50660.13491129302860674No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC50110.1334466027174No Hit
GCCTACATGGAGCTGAGGAGCCTGAGATCTGACGACACGGCCGTGTATTA49970.13307377245636554No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC47240.12580358236619388No Hit
CAGTAATACACGGCCGTGTCGTCAGATCTCAGGCTCCTCAGCTCCATGTA47040.12527096770757326No Hit
TAGTAGCACTTTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTC46870.12481824524774571No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATAACCTTGTCTCCT46180.12298072467550453No Hit
GTGTATTACTGTGCGAGGTATACCTCCAATACTTGCTTTGACTACTGGGG45690.12167581876188396No Hit
ACCTACACCTGCGTGGTGGCCCATGAGGCCCTGCCCAACAGGGTCACCGA45230.12045080504705649No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG44630.11885296107119461No Hit
GTCTGGAGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA44360.11813393128205674No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC44000.11717522489653961No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC43970.11709533269774651No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC43430.11565727311947079No Hit
GTATTGGAGGTATACCTCGCACAGTAATACACGGCCGTGTCGTCAGATCT43220.11509802772791913No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA43120.11483172039860881No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT42850.11411269060947096No Hit
CATGTAGGCTGTGCTCGTGGATGTGTCTGTGGTCATGGTGACTCTGCCCT42270.11256810809947111No Hit
CCCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGCAAG41940.11168929391274707No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG41600.11078384899309199No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG41570.11070395679429888No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC40920.10897295915378183No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA40560.10801425276826468No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT39640.10556422533860978No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA39350.10479193408360984No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA39170.10431258089085128No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG38710.10308756717602383No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAA38410.10228864518809287No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38320.10204896859171358No Hit
ATACACGGCCGTGTCGTCAGATCTCAGGCTCCTCAGCTCCATGTAGGCTG38300.10199570712585151No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT38140.101569615398955No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT38130.10154298466602397No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)