FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106973

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106973
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3739595
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCATCATGGGATGACAGCCTGAATGGTTGGGTGTTCGG129360.34591981217217377No Hit
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAGGTCAACCTCAGTGAGT122520.3276290614357972No Hit
GTAATAATCAGTCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG120470.3221471843876142No Hit
ATCTATCACAGTGGGTCCATCAACTACAACCCGTCCCTCAGGAGTCGAGC83250.22261768988353017No Hit
GTGTATTACTGTGCGCGAGGGGTCGCAGAGGTCAGCACTGGCTGGTACGA81520.21799152047213669No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCC71410.190956507322317No Hit
GTATTACTGTGCGCGAGGGGTCGCAGAGGTCAGCACTGGCTGGTACGAAG63720.17039278317571824No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT59620.1594290290793522No Hit
CAGTAATACACGGCCGTGTCTGCGGCGGTCACAGAGGTCAACCTCAGTGA57150.15282403575788286No Hit
ACTACAACCCGTCCCTCAGGAGTCGAGCCACCCTCTCGATGGACAAGTCC57100.1526903314396345No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT53670.14351821520779656No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG52760.1410847966156763No Hit
GTCTTACCTGGTGGAGTTGGGTCCGCCAGCCCCCAGGGAAGGGGCTGGAG51770.13843745111435865No Hit
TTCTGAGACTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTC51120.13669929497712988No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA51030.1364586272042828No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA48270.129078148836973No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA48220.12894444451872464No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT47610.12731325183609454No Hit
TTATTACTGTGCATCATGGGATGACAGCCTGAATGGTTGGGTGTTCGGCG47330.1265645076539037No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAACCATTCAGG46040.12311493624309584No Hit
CTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCT44320.11851550769535205No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA44140.11803417214965792No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGAGTCAGCCACCCTCAGCGTCG43430.11613557083053111No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC42830.1145311190115507No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA42350.1132475575563664No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT42190.11281970373797164No Hit
GTCCTGGGCCCAGTCTGTGCTGAGTCAGCCACCCTCAGCGTCGGGGACCC41480.11092110241884483No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT41110.10993169046380691No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG40970.10955731837271147No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA40850.1092364280089154No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC40260.1076587170535847No Hit
GGTCAGCACTGGCTGGTACGAAGGTGCTTTTGATATCTGGGGCCAAGGGA39430.10543922537066179No Hit
GTGCTGACCTCTGCGACCCCTCGCGCACAGTAATACACGGCCGTGTCTGC38730.10356736491518466No Hit
GTGATAGATTTCCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGA38690.10346040146058597No Hit
CTATCACAGTGGGTCCATCAACTACAACCCGTCCCTCAGGAGTCGAGCCA38390.10265817555109577No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG37990.10158854100510883No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG37730.10089327855021735No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG37470.10019801609532583No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)