FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005106976

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106976
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3724165
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG138090.37079452709533545No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGCTCAGCTTCAGGGAGA109800.2948311903473665No Hit
GTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGCCCAGGGTGG108530.29142102994899527No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT90820.24386674596855942No Hit
ACATGGGATAGCAGCCTGAGTGCTGTGGTCTTCGGCGGAGGGACCGAGCT87970.2362140238147343No Hit
GATTATTACTGCGCAACATGGGATAGCAGCCTGAGTGCTGTGGTCTTCGG86390.23197146205928043No Hit
GTGTATTACTGTGCGAGGGGCTATGGTTCGGGGAGTTACCCGTACTACTA78890.2118327195492144No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT78860.21175216457917412No Hit
GGGTATATCTATTACATTGGGAGCACCAACTACAACCCCTCCCTCAAGAG77760.20879848234436446No Hit
ATCTATTACATTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGT77680.20858366909092377No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA76380.2050929537225123No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC75250.20205871651766236No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC73430.19717171500188632No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA72930.19582913216788195No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTCGGTCCCTCCGCCGAAGACCAC71470.1919087902925891No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA71180.1911300922488665No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT70110.1882569649840971No Hit
GTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCC69770.1873440086569741No Hit
ATCCCATGTTGCGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGG68660.18436347476548434No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA67200.18044313289019148No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA64730.17381077369020975No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCC63300.16997098678495717No Hit
CTATTACATTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGTCA61920.166265458163105No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG60390.16215715469105157No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC60040.1612173467072485No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA58700.15761922471211667No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG58170.15619608690807202No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC57780.1551488722975486No Hit
GATATACCCAATCCACTCCAGTCCCTTCCCTGGGGGCTGCCGGATCCAGC57460.15428961928378576No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA54990.147657260083804No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT54260.1456970891461576No Hit
CCCAGGAACAGCCCCCAAACTCCTCATTTATGACGATAATAAGCGACCCT53770.14438135796883328No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG53710.14422024802875275No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC53460.14354895661175057No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT53420.1434415499850302No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA52300.14043416443686035No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT51270.13766844379881127No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA51240.13758788882877102No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT49510.13294255222311577No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC48970.13149256276239102No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT48860.13119719453891007No Hit
GTATTACTGTGCGAGGGGCTATGGTTCGGGGAGTTACCCGTACTACTACT48550.13036479318182734No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG48360.12985461170490567No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA48310.12972035342150523No Hit
TCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC47180.12668611621665526No Hit
ATGTAATAGATATACCCAATCCACTCCAGTCCCTTCCCTGGGGGCTGCCG45620.12249725777456154No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA44680.11997320204663327No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC44510.11951672388307177No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT44200.11868432252598905No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA43640.11718062975190412No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT43530.11688526152842316No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG43380.11648248667822184No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT42980.1154084204110183No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG42920.1152473104709378No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC42260.11347510113005198No Hit
CTACTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCT42220.11336769450333163No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC42050.11291121633977012No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC42010.11280380971304976No Hit
CAGTAATACACGGCCGTGTCCGCAGCGGTCACAGAGCTCAGCTTCAGGGA41920.11256214480292898No Hit
GTAATAGATATACCCAATCCACTCCAGTCCCTTCCCTGGGGGCTGCCGGA41270.11081678711872325No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC40850.10968901753815956No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT40240.1080510664806742No Hit
GGATAGCAGCCTGAGTGCTGTGGTCTTCGGCGGAGGGACCGAGCTGACCG40180.10788995654059366No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGG39720.10665478033330962No Hit
CCGTAGTAGTAGTACGGGTAACTCCCCGAACCATAGCCCCTCGCACAGTA39720.10665478033330962No Hit
GGATTGGGTATATCTATTACATTGGGAGCACCAACTACAACCCCTCCCTC39330.10560756572278618No Hit
GTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCCTCC39130.10507053258918442No Hit
GTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCAC38520.10343258153169906No Hit
ACCCAGGACGGTCAGCTCGGTCCCTCCGCCGAAGACCACAGCACTCAGGC38480.10332517490497871No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC38020.10208999869769465No Hit
CTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCA37650.1010964874005314No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)