FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106987

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106987
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3622809
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA59130.16321589131527497No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT56370.15559749354713429No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC52920.1460744963369584No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC49780.1374071887311752No Hit
GTATTATTGTGTCACACAGGAGGAGCGATTCAGTGACTTCCAATGGGTGT48880.13492292858939017No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT46810.12920913026328465No Hit
CCCTTAGAGTCTCCTGTGCAGCGTCTGGATTCGCCTTGAGTAACACCTGG44830.12374375795135763No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA44550.12297087701835785No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC44540.12294327412789358No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT41160.11361349715096765No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG40380.111460471694754No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT40220.11101882544732554No Hit
GTGTATTATTGTGTCACACAGGAGGAGCGATTCAGTGACTTCCAATGGGT36850.1017166513608639No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA36500.1007505501946142No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)