FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005106991

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106991
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4240063
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTATGGTGGTGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCC154390.364121948188034No Hit
CCATAGAAGAGTAGGCAGTAATACTCAGCCTCGTCCTCAGGCTGCACACC151900.358249393935892No Hit
GTCTCTGGTGTCTCCATCAATGGTTACTACTGGACCTGGATCCGGCAGCC120420.2840052140734701No Hit
GATTTATAGTACCACTAACAAACACTCCTGGACCCCTGCCCGGTTCTCAG114650.2703969257060567No Hit
CTATAAATCAATGCCCTGGGTGCTTGTCCAGGTTTCTGCTGGAACCAGTT109860.25909992375113294No Hit
GTACTGGAGCAGTCACCAGTGCTTACTCTCCAAACTGGTTCCAGCAGAAA102680.242166213096362No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT100520.23707194916679303No Hit
GTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCAC100180.23627007428899052No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA93250.2199259775149567No Hit
GGTTATATCTATTCCAGTGGGGGCACCAACTCCAACCCCTCCCTCAAGAG92690.21860524242210555No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCC91280.215279820134748No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGCTCCTCCTCACTTGCT90730.21398266959712628No Hit
GTCCAGGAGTGTTTGTTAGTGGTACTATAAATCAATGCCCTGGGTGCTTG90500.2134402248268481No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT88910.20969028054535982No Hit
CTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTG87760.20697805669396893No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA87170.20558656793542926No Hit
TCTGTAGACACATCCAAGAACCAGTTCTCCCTGAGGCTGAGCTCTGTGAC87010.20520921505175746No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGCACCACCA85930.202662083086973No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA83160.19612916128840538No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC76380.1801388328428139No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC75910.17903035874702805No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC74230.1750681534684744No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA73270.17280403616644374No Hit
CTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTTCCA73260.17278045161121428No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT71480.16858240078036577No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA71120.16773335679210427No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT70630.16657771358585946No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT70620.16655412903062997No Hit
GTAATACTCAGCCTCGTCCTCAGGCTGCACACCTGACAGTGTCAGGGCAG69730.1644551036152057No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG68740.1621202326474866No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT68080.16056365200234055No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG67820.15995045356637388No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG67460.15910140957811242No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG66900.15778067448526117No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA66390.1565778621685574No Hit
TCCTCAGGCTGCACACCTGACAGTGTCAGGGCAGCTTTGCCCCCAAGGAG65900.1554222189623126No Hit
GTATTACTGCCTACTCTTCTATGGTGGTGCTTGGGTGTTCGGCGGAGGGA65440.15433732942175624No Hit
TTCTCAGACTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAG65370.15417223753514983No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT64740.1526864105556922No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG63230.14912514271603983No Hit
GAGTATTACTGCCTACTCTTCTATGGTGGTGCTTGGGTGTTCGGCGGAGG62820.14815817595163092No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC62430.14723837829768097No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA60760.14329975757435678No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA60070.14167242326352225No Hit
GGCCTGGACTCCTCTCTTTCTGCTCCTCCTCACTTGCTGCCCAGGGTCCA58940.13900736852259035No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT57390.13535176246202002No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT56620.1335357517093496No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC56510.13327632160182526No Hit
GTCTACAGATATGGTGACTCGACTCTTGAGGGAGGGGTTGGAGTTGGTGC56380.13296972238384192No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC56240.13263953861062913No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCT55930.13190841739851505No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACA55330.13049334408474592No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA55200.1301867448667626No Hit
CACCTGCACTGTCTCTGGTGTCTCCATCAATGGTTACTACTGGACCTGGA54570.12870091788730498No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA53390.12591794037022563No Hit
GTGCTTACTCTCCAAACTGGTTCCAGCAGAAACCTGGACAAGCACCCAGG52430.12365382306819497No Hit
ATCTATTCCAGTGGGGGCACCAACTCCAACCCCTCCCTCAAGAGTCGAGT52070.12280477907993348No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG51280.12094159921680409No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA51120.12056424633313233No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT50890.12002180156285414No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA50680.11952652590303493No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG49680.11716807038008634No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC49290.11624827272613639No Hit
CTACTGGACCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTG48410.11417283186594161No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG48150.11355963342997498No Hit
CTTTCTGAGAGTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGT47760.11263983577602503No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGG45710.10780500195398039No Hit
GTTTGTTAGTGGTACTATAAATCAATGCCCTGGGTGCTTGTCCAGGTTTC44990.1061069139774574No Hit
GGTTACTACTGGACCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTG44870.10582389931470357No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGG44700.10542296187580232No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGT44070.1039371348963447No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC44050.10388996578588573No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT43970.10370128934404983No Hit
CTCCAAACTGGTTCCAGCAGAAACCTGGACAAGCACCCAGGGCATTGATT43410.10238055425119863No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGC43180.10183810948092044No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA42890.10115415737926535No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGA42890.10115415737926535No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT42610.10049378983283976No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC42460.10014002150439745No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)