FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106992

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106992
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4240063
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTATGGTGGTGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCC168570.3975648475034451No Hit
GATTTATAGTACCACTAACAAACACTCCTGGACCCCTGCCCGGTTCTCAG146710.3460090097717887No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG139010.32784890224508456No Hit
CTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTG130720.30829730595984073No Hit
CCATAGAAGAGTAGGCAGTAATACTCAGCCTCGTCCTCAGGCTGCACACC127860.3015521231642077No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGCTCCTCCTCACTTGCT117600.2773543694987551No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT107200.25282643206008965No Hit
GTACTGGAGCAGTCACCAGTGCTTACTCTCCAAACTGGTTCCAGCAGAAA106760.2517887116299923No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCC106470.25110475952833716No Hit
TCTGTAGACACATCCAAGAACCAGTTCTCCCTGAGGCTGAGCTCTGTGAC101910.24035020234369162No Hit
CTATAAATCAATGCCCTGGGTGCTTGTCCAGGTTTCTGCTGGAACCAGTT100640.23735496382954685No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT91140.21494963636153522No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA87230.2057280752668062No Hit
GGTTATATCTATTCCAGTGGGGGCACCAACTCCAACCCCTCCCTCAAGAG86950.20506770772038058No Hit
GTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCAC85500.20164794721210508No Hit
GTCTCTGGTGTCTCCATCAATGGTTACTACTGGACCTGGATCCGGCAGCC83500.19693103616620788No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT82420.19438390420142343No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC79970.18860568817019938No Hit
GTCCAGGAGTGTTTGTTAGTGGTACTATAAATCAATGCCCTGGGTGCTTG79600.1877330596267084No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA78610.18539818865898927No Hit
GGCCTGGACTCCTCTCTTTCTGCTCCTCCTCACTTGCTGCCCAGGGTCCA76140.17957280351730623No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGCACCACCA75070.17704925610775124No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC74920.17669548777930894No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA74840.17650681133747304No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA74770.17634171945086666No Hit
CTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTTCCA74500.17570493645967053No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA72100.1700446432045939No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT71520.16867673900128372No Hit
GTATTACTGCCTACTCTTCTATGGTGGTGCTTGGGTGTTCGGCGGAGGGA69520.16395982795538652No Hit
GTCTACAGATATGGTGACTCGACTCTTGAGGGAGGGGTTGGAGTTGGTGC68680.16197872531610968No Hit
GAGTATTACTGCCTACTCTTCTATGGTGGTGCTTGGGTGTTCGGCGGAGG68150.16072874388894692No Hit
GATATAACCAATCCACTCCAGTCCCTTCCCTGGGGGCTGCCGGATCCAGG66290.15634201661626254No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA65640.15480902052634596No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT65090.1535118699887242No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG64090.15115341446577563No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG63730.15030437047751413No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC62920.14839402150392578No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC62830.1481817605068604No Hit
TTCTCAGACTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAG60680.1431110811325209No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT59960.14141299315599792No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT58560.13811115542386987No Hit
GTAATACTCAGCCTCGTCCTCAGGCTGCACACCTGACAGTGTCAGGGCAG58320.13754512609836222No Hit
CCTCTCTTTCTGCTCCTCCTCACTTGCTGCCCAGGGTCCAATTCTCAGAC57350.13525742424110207No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG57350.13525742424110207No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT56820.1340074428139393No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA56680.13367725904072653No Hit
CTAACAAACACTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGG56230.13261595405539964No Hit
TCCTCAGGCTGCACACCTGACAGTGTCAGGGCAGCTTTGCCCCCAAGGAG55890.1318140791775971No Hit
GTGCTTACTCTCCAAACTGGTTCCAGCAGAAACCTGGACAAGCACCCAGG55400.1306584359713523No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA55080.12990373020400878No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT53930.12719150635261786No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA53790.12686132257940508No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA52780.12447928250122699No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA52510.12384249951003087No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG52150.12299345552176937No Hit
CTCCAAACTGGTTCCAGCAGAAACCTGGACAAGCACCCAGGGCATTGATT50160.11830012903110167No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC50160.11830012903110167No Hit
GTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACACTGTCAGGTG50010.11794636070265936No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG49630.1170501476039389No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC49580.11693222482779148No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC49280.11622468817090688No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG49040.11565865884539923No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC48840.11518696774080951No Hit
TCCAATATCAGCACCATGGCCTGGACTCCTCTCTTTCTGCTCCTCCTCAC48340.11400773997933521No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT48220.1137247253165814No Hit
ATCTATTCCAGTGGGGGCACCAACTCCAACCCCTCCCTCAAGAGTCGAGT47920.1130171886596968No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC47590.11223889833712378No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA46900.11061156402628923No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC46270.10912573704683162No Hit
ATATACGGCCGTGTCCGCAGCGGTCACAGAGCTCAGCCTCAGGGAGAACT46260.10910215249160211No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT45000.1061304985326869No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT45000.1061304985326869No Hit
GCCTGAGGACGAGGCTGAGTATTACTGCCTACTCTTCTATGGTGGTGCTT44870.10582389931470357No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT44550.10506919354736004No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACA44460.10485693255029464No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC44130.10407864222772162No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG43130.10172018670477302No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)