FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005107011

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107011
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3130757
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124040.3961981079975227No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT80300.2564874884892056No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79560.2541238428916712No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63510.20285828635055353No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA60980.1947771736995238No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA51240.16366648705089537No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCGCGTATGCCGT49690.1587156077587625No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA49110.1568630206688031No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT43920.14028556032933887RNA PCR Primer, Index 39 (95% over 23bp)
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA42850.13686785655993103No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA41160.13146980107367004No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG39430.12594398096051532No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA35420.11313557711441673No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG35300.11275228323373548No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34980.11173016621858547No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAA34110.10895128558364639No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC33320.10642793420249479No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32140.10265887770912914No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)