FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005107037

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107037
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1959227
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC68920.351771387389006No Hit
ATGTATTACTGTGCGAGGTCCGGATTCGAGTATAGCAGCTCGTCCCTAGG64060.32696568595675746No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAAATGGTCAGGGAGG57380.2928706066218973No Hit
GGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAA53880.2750064183476442No Hit
GATTATTACTGCTGCTCATATGCAGGCAGCTACACTTCCCCTGTGGTATT53620.2736793643615569No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA49250.25137464928770376No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT47340.24162590654375427No Hit
GTAATACATGGCGGTGTCCGAGGCCTTCAGGCTGCTCCACTGCAGGTAGG46630.23800202835097722No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA45490.23218340702736334No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT44160.22539501548314717No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC41260.21059325948448038No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAG40250.2054381651539102No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTT39910.20370278686441132No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG37570.191759300989625No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA37040.1890541524795238No Hit
GTATTACTGTGCGAGGTCCGGATTCGAGTATAGCAGCTCGTCCCTAGGCT35820.18282720685249845No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG35620.1818063960939697No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA34630.17675338283925243No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATAACTATGTCTCCT34210.17460968024634205No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT33570.17134308581905006No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT33220.16955666699162475No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG32690.16685151848152358No Hit
GTCATTAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTC32310.16491197804031896No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA32290.1648098969644661No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCT31590.1612370593096155No Hit
CTCTTATACCAACTACAGCCCGTCCTTCCAAGGCCACGTCACCATCTCAG31560.16108393769583618No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAGGGGAAGTG31550.16103289715790972No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA31260.15955272155804304No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC29750.15184560033115102No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC29380.14995710042787283No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA29130.14868108697971189No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT29110.14857900590385903No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG28950.14776235729703605No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC28740.14669050600058084No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG28270.1442916007180383No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA27940.14260726296646586No Hit
CCTGTAAGGGTTCTGGATACAGGTTTTCCAGCTACTGGATCAGCTGGGTG27490.14031043875977617No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC27200.13883026315990948No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG26920.13740112809796926No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC26900.13729904702211637No Hit
GTTATAACCACCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGACTG26890.13724800648418994No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATA26890.13724800648418994No Hit
GGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGATGTC26380.13464493904994165No Hit
TCCCTAGGCTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTC26230.13387933098104507No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT26170.13357308775348645No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT26100.1332158039880014No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC25830.1318377094639876No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG25820.13178666892606114No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC25700.1311741824709439No Hit
GTATAAGAGTCACTAGGATCAATCCTCCCCATCCACTCCAGGCCTTTCCC25550.13040857440204734No Hit
GTCTGGAGCAGAGGTGAAAAAGCCCGGGGAGTCTCTGAGGATCTCCTGTA25550.13040857440204734No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA25420.12974504740900364No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG25280.1290304798780335No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTC25150.12836695288498984No Hit
TCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTG24820.12668261513341741No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT23920.12208896672003806No Hit
GCTCATATGCAGGCAGCTACACTTCCCCTGTGGTATTCGGCGGAGGGACC23750.12122127757528862No Hit
TTTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCTGCTCATAT23710.12101711542358287No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA23680.12086399380980355No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT23500.1199452641271277No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGG22510.1148922508724104No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT22440.11453496710692533No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG22400.11433080495521959No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC22350.11407560226558741No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC22320.1139224806518081No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT22140.1130037509691322No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG21960.11208502128645635No Hit
TTTATGATGTCATTAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGC21540.10994131869354598No Hit
CATATGAGCAGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAA21160.10800177825234136No Hit
CTTAATGACATCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGCTGTT20790.10611327834906317No Hit
CTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGA20770.1060111972732103No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAGGGGAAGTGT20600.10514350812846086No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGA20560.10493934597675511No Hit
TCTCCAAGGAGTCTGTGCCGAAGTGCAGCTGGTGCAGTCTGGAGCAGAGG20420.10422477844578498No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)