FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005107038

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107038
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1959227
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG78170.3989838849709605No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC59080.30154749806939163No Hit
GATTATTACTGCTGCTCATATGCAGGCAGCTACACTTCCCCTGTGGTATT56150.2865926204569455No Hit
ATGTATTACTGTGCGAGGTCCGGATTCGAGTATAGCAGCTCGTCCCTAGG55010.2807739991333317No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA48930.2497413520740578No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAAATGGTCAGGGAGG48540.24775077109492671No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT48270.2463726765709129No Hit
GGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAA47680.24336128483325314No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA47100.24040093363351975No Hit
GTAATACATGGCGGTGTCCGAGGCCTTCAGGCTGCTCCACTGCAGGTAGG45510.23228548810321623No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT44480.2270283126967932No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG43520.2221284210558552No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAGGGGAAGTG40490.20666313806414469No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC40480.20661209752621826No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA37830.19308635497571236No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTT37160.18966663893464106No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT35680.18211263932152835No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA35250.1799178961906915No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG33580.1713941263569765No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG32860.16771920762627301No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA32810.16746400493664082No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAG32660.16669839686774426No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC32590.1663411131022592No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT32020.16343180244045227No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCT30550.155928843365266No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC30370.15501011368259013No Hit
GTATTACTGTGCGAGGTCCGGATTCGAGTATAGCAGCTCGTCCCTAGGCT30340.15485699206881082No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA30320.15475491099295793No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA30200.1541424245378407No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC30150.1538872218482085No Hit
GTCATTAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTC29970.15296849216553263No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATAACTATGTCTCCT29450.15031438419335788No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC29020.1481196410625211No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC28890.1474561140694774No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG28810.14704778976606592No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA26940.1375032091738221No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAGGGGAAGTGT26520.13535950658091178No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT26380.13464493904994165No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA26230.13387933098104507No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA26060.13301164183629563No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG25960.13250123645703127No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTC25960.13250123645703127No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT25840.13188875000191402No Hit
CCTGTAAGGGTTCTGGATACAGGTTTTCCAGCTACTGGATCAGCTGGGTG25680.131072101395091No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC25240.12882631772632777No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC25210.12867319611254846No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT24690.12601908814037374No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT24390.12448787200258062No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA24390.12448787200258062No Hit
GTCTGGAGCAGAGGTGAAAAAGCCCGGGGAGTCTCTGAGGATCTCCTGTA24050.12275249371308174No Hit
CTCTTATACCAACTACAGCCCGTCCTTCCAAGGCCACGTCACCATCTCAG23850.12173168295455299No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC23720.12106815596150931No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT23430.11958798036164262No Hit
TCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTG23260.1187202912168932No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA23200.11841404798933458No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATA23090.11785260207214376No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC22970.1172401156170265No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC22710.11591306163093915No Hit
GGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGATGTC22530.11499433194826326No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGG22420.11443288603107246No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG22370.11417768334144028No Hit
TTTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCTGCTCATAT22280.11371831850010233No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT22190.1132589536587644No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT22180.11320791312083797No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT22120.11290166989327934No Hit
GTTATAACCACCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGACTG22070.11264646720364715No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG21920.11188085913475058No Hit
GTATAAGAGTCACTAGGATCAATCCTCCCCATCCACTCCAGGCCTTTCCC21800.11126837267963334No Hit
GTGTAGCTGCCTGCATATGAGCAGCAGTAATAATCAGCCTCATCCTCAGC21190.10815489986612067No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGT20450.1043779000595643No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG20320.10371437306652062No Hit
CTTAATGACATCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGCTGTT20290.10356125145274131No Hit
TCCCTAGGCTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTC20250.10335708930103557No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC20240.10330604876310913No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCC20200.10310188661140336No Hit
TTTATGATGTCATTAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGC20160.10289772445969762No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC20000.10208107585287464No Hit
TCTCCAAGGAGTCTGTGCCGAAGTGCAGCTGGTGCAGTCTGGAGCAGAGG19980.10197899477702174No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA19800.10106026509434589No Hit
GCTCATATGCAGGCAGCTACACTTCCCCTGTGGTATTCGGCGGAGGGACC19740.10075402186678725No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC19650.10029465702544932No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)