FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005107045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2485145
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT65520.2636465880260508No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC59240.2383764327634806No Hit
GTAATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATACA46220.18598512360445768No Hit
AATAAATACTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGA42530.17113689543266086No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG42330.17033211341792934No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG39260.15797870949180026No Hit
ACAGTACCCTATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGA35770.143935263334735No Hit
GTGTATTACTGTGCGAAGGATCGCGGGTTTGGTAGGATAGCAGCAGCTGG35260.1418830691971696No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA35060.14107828718243803No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAG33780.13592768228815624No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT32950.13258783692702036No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG32710.1316220985093425No Hit
GAGTTACAGTACCCTATTCACTTTCGGCCCTGGGACCAAAGTGGATATCA30540.12289021364950536No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29760.11975156379205239No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC28730.11560693641618498No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG28550.11488263260292658No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28400.11427904609187793No Hit
ATACTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAACT27940.11242804745799542No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTG27080.10896748479464981No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG27020.10872605019023034No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT26610.1070762470600307No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT26490.10659337785119177No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA26020.10470214011657267No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA25830.10393759720257771No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT25800.103816879900368No Hit
ATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGTTCTTGATC24870.10007464353186636No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)