FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005107063

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107063
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3771192
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACATGCAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTATATTA132550.35148038073903426No Hit
GTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCT124490.33010782797587607No Hit
GTAATATACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTGCATGTAGA121730.32278918707931076No Hit
GTATATTACTGTGCGAGAGATTCCCAGGGGTTTTTTGGAGTCAACTACTA108250.28704452067144814No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT105600.28001756473815176No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC104050.2759074584375444No Hit
GGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAATAATCAGC72620.1925651093871646No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC67910.1800756895962868No Hit
TTATTACTGCATGCAAGCTCTACAAACTCCTAAAACGTTCGGCCAAGGGA62680.16620739543359234No Hit
CATGTAGACTGTGCTCGTGGACGTGTCCCTGGTCATGGTGACTCTGTCCT61100.16201773868845712No Hit
ATTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATG59650.15817280053627608No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT58450.1549907827551607No Hit
TTCCAGGACAGAGTCACCATGACCAGGGACACGTCCACGAGCACAGTCTA57230.15175573134436007No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCT56630.1501647224538024No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCA55910.1482555117851332No Hit
ATCCATTACTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC55790.14793731000702165No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT54750.14517956126338832No Hit
CTACTACGGTGTGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCT54360.14414540548452587No Hit
CCCTTATGATTTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG53020.1405921522956137No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC52610.13950496288706596No Hit
GGATACACCTTCACCAATTATTATATGCACTGGGTGCGACAGGCCCCTGG51240.1358721592536259No Hit
CTACAGAAGCCTCTGAGAGGAAAGTTCTTCACCATGGACTGGACCTGGAG51020.1352887893270881No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG49280.13067486354447083No Hit
GCCGTATATTACTGTGCGAGAGATTCCCAGGGGTTTTTTGGAGTCAACTA47890.12698902628134554No Hit
GCATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGC47830.12682992539228977No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA47720.12653824042902084No Hit
CCGTAGTAGTAGTAGTTGACTCCAAAAAACCCCTGGGAATCTCTCGCACA47260.12531846694625995No Hit
ATATACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTGCATGTAGACTG46160.12240161731357088No Hit
GGAATAATCAGCCCTAGTGGTCGTAGCACAAGCTACGCACAGAAGTTCCA42220.11195399226557544No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC41710.11060163470860143No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAAGTGGTACGT41420.10983264707816522No Hit
GTTTATTACTGCATGCAAGCTCTACAAACTCCTAAAACGTTCGGCCAAGG40480.10734006648295817No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA40340.10696883107516138No Hit
GTAATAAACTCCAATATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAA40320.10691579744547614No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG39680.10521872129554795No Hit
CAGTAATAAACTCCAATATCCTCAGCCTCCACTCTGCTGATTTTCAGTGT39530.10482096907290851No Hit
ATATTACTGTGCGAGAGATTCCCAGGGGTTTTTTGGAGTCAACTACTACT39160.10383984692373127No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGAATAATCAGCCCTAGTGGTCGT39090.10365422921983287No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)