FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005107077

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107077
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4033943
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTATTATAGTGGGAGTACCTACTACAACCCGTCCCAACCGTCCCTCAA227410.5637412328334833No Hit
GTAGTACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTCCAGGGAGA126540.31368812102699517No Hit
CTATTATAGTGGGAGTACCTACTACAACCCGTCCCAACCGTCCCTCAAGA116830.2896173793234064No Hit
ATAATAGATAGTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGA112200.27813977540089185No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC93660.23217978042823112No Hit
GTGTACTACTGTGCGAGACTAGAGGACCTCTGGGGGTCCTCTGACTACTG92360.22895712705905857No Hit
GTCTCTGGTGGCTCCATCAGCACTAGTAGTTACTACTGGGGCTGGATCCG89170.2210492314839352No Hit
ACCTACTACAACCCGTCCCAACCGTCCCTCAAGAGTCGAGTCACCATGTC83980.20818340764854637No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA71260.1766509839132581No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA71240.1766014046306554No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACAATGCCAGTG67080.16628891384930328No Hit
ACTACAACCCGTCCCAACCGTCCCTCAAGAGTCGAGTCACCATGTCCGTA66930.16591706922978336No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT65140.16147972343684577No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT64390.15962050033924624No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC62200.15419156889425556No Hit
GTACTTGATGAAGCTTTACAGTGATGGCGGCCACATCAAGGGGGACGGGA60490.1499525402317286No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG58000.14377991954769812No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA57760.14318496815646625No Hit
ATTATAGTGGGAGTACCTACTACAACCCGTCCCAACCGTCCCTCAAGAGT57600.14278833389564505No Hit
CATCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGG57360.14219338250441318No Hit
CTATAATAGATAGTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCG56900.14105305900455212No Hit
GACTATTACTGTCAGACCTGGGGCACTGGCATTGTGGTTTTCGGCGGAGG56510.14008626299380036No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC56370.1397392080155818No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT56030.13889636021133667No Hit
GTAATAGTCAGCCTCATCCTCAGACTGGAGGCTGGAGATGGTGAGGTAGC54280.13455817298360437No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG52410.12992251006025618No Hit
CTATTACTGTCAGACCTGGGGCACTGGCATTGTGGTTTTCGGCGGAGGGA52180.12935234831032566No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC52130.12922840010381903No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA50890.12615448458245443No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG50560.12533642641951065No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCAC50400.1249397921586894No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT50360.1248406335934841No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA49790.12342762403930845No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG49720.12325409655019916No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT49260.12211377305033809No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT48240.11958522963760271No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG47890.11871759219205626No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA47620.11804827187692042No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA46120.11432982568172134No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA45700.11328866074706559No Hit
GCTTTACAGTGATGGCGGCCACATCAAGGGGGACGGGATCCCTGATCGCT44760.11095843446474084No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA44710.1108344862582342No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACAATGCCAGTGC43100.1068433540087205No Hit
GTACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTCCAGGGAGAACT42680.10580218907406476No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG42610.10562866158495546No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC42540.10545513409584617No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG42470.10528160660673688No Hit
ACTCTGAGCAGTGGGCACAGCAACTACGCCATCGCATGGCATCAGCAGCA42270.10478581378071035No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT41780.10357112135694531No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCC41500.10287701140050814No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT41480.10282743211790547No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC41340.1024803771396869No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA40610.10067073332469001No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)