FastQCFastQC Report
Sun 7 Aug 2022
EGAF00005107081

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107081
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3985257
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT154620.3879799972749562No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC150070.3765629167704868No Hit
GTCTCTGGTGGCTCCATCAATAATTACTACTGGAATTGGATCCGGCAGCC144450.36246094041112026No Hit
CCATTATACTGGGAGCACAAATTACAACCCCTCCCTCAAGAGTCGAGTCA139190.3492622934982612No Hit
GGTTAATAGTTACCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAAC129390.324671658565558No Hit
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA127390.31965316164051655No Hit
GTGTATTACTGTGCGACCATCCGAAAAGGACCTGTTTTCGGGGAGCCACA116180.2915244863756591No Hit
CTATTAACCTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCT105940.26582978211944674No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAGCAG86330.21662341976941513No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT79850.20036348973228074No Hit
GGATATATCCATTATACTGGGAGCACAAATTACAACCCCTCCCTCAAGAG75370.18912205662018786No Hit
GTATTACTGTGCGACCATCCGAAAAGGACCTGTTTTCGGGGAGCCACATT73480.18437957702602367No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA72250.18129320141712316No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG71750.18003857718586277No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA70910.17793080847734538No Hit
GGATTGGATATATCCATTATACTGGGAGCACAAATTACAACCCCTCCCTC70050.17577285479957752No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG67860.17027760066665715No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC67770.17005176830503027No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC67580.1695750110971513No Hit
TATTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAACTCTTGAT66570.16704067015000537No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT66170.16603697076499707No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG65120.1634022598793503No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT63130.15840885543893404No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT61390.15404276311414797No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG61370.15399257814489756No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC60650.15218591925188263No Hit
GATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCG59380.1489991737044813No Hit
CTCTTGATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTT58620.1470921448729655No Hit
TTACTACCTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG57840.14513493107219935No Hit
ATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCCGT57190.1435039195715609No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG56800.1425253126711778No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG56630.14209874043254928No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC56190.14099467110904015No Hit
ACTTATTACTGTCAACAGGTTAATAGTTACCTCACTTTCGGCGGAGGGAC55750.13989060178553103No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC55570.13943893706227728No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC54740.13735626083838506No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA51930.13030527265870182No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT51290.12869935364268856No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC50540.12681741729579799No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA49940.12531186821828555No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT49550.12433326131790247No Hit
TTATTACTGTCAACAGGTTAATAGTTACCTCACTTTCGGCGGAGGGACCA49300.12370594920227226No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAATAATTACTACTGGAATTGGA48900.12270224981726398No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG46630.1170062558073419No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA46420.11647931363021255No Hit
TATCAGTAGACACGCCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTG46070.1156010766683303No Hit
AATTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGCC45530.1142460824985691No Hit
AGGTAGTAATGTGGCTCCCCGAAAACAGGTCCTTTTCGGATGGTCGCACA45290.11364386286756413No Hit
CATTACTACCTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTC45270.1135936778983137No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG45140.11326747559818602No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG45010.11294127329805831No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA44840.11251470105942979No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCT44250.11103424446654256No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC43850.11003054508153427No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA42980.10784749891914122No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT42870.10757148158826393No Hit
CAGTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGA42850.10752129661901354No Hit
GTAGTAATGTGGCTCCCCGAAAACAGGTCCTTTTCGGATGGTCGCACAGT42660.10704453941113459No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC42440.10649250474938003No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC42370.10631685735700358No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC41770.10481130827949114No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT41400.10388288634835846No Hit
CTTTCTGAGAGTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGT41040.10297955690185101No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT40870.10255298466322246No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG40810.10240242975547123No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)