FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005107091

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107091
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5454565
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTAATTACTCCATCAGAAATTATTACTTCTGGGGCTGGATCCGGCA216880.3976119085573277No Hit
CTATAACCCGTCCCTCAAGAATCGACTCACCATGTCAATAGACACGTCCA174570.32004385317619277No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC144380.26469571817367654No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT138530.25397075660478885No Hit
GGTGTACACCCTGCCCCCATCCCAGGAGGAGATGACCAAGAACCAGGTCA112200.20569926291097457No Hit
CTATTATTGTGCGAGAGGACGTCGACGGGGAGACTACGACTCTTGGGGCC110920.20335260465316665No Hit
CAATAATAGAAGGCCGTGTCTGCGGCGGTCACAGAGGTCAGGTTCAGGGA107430.19695429424711228No Hit
GTGTACACCTGTGGCTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT92900.1703160563674647No Hit
GTTTATTACTGTCAACAATTTTTTACTACTCCATTCACTTTCGGCCCTGG92500.16958272566189972No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC91710.1681343975184089No Hit
CTCCTAAGCTGCTCATTCACTGGGCATCTATGCGGGAATCCGGGGTCCCT89780.16459607686405792No Hit
CACCTGCACTGTCTCTAATTACTCCATCAGAAATTATTACTTCTGGGGCT86390.15838109913439477No Hit
GTGGTGGACGTGAGCCAGGAAGACCCCGAGGTCCAGTTCAACTGGTACGT82820.1518361225872274No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC82270.15082779286707557No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT74970.13744450749051482No Hit
ATTAGAGACAGTGCAGGTGAGGGACAGGGTCTCCGAAGGCCTCACCAGTC71040.13023953330833898No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC70730.12967120201152613No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT67530.12380455636700634No Hit
ATAGAAGGCCGTGTCTGCGGCGGTCACAGAGGTCAGGTTCAGGGAGAACT66350.12164123078558968No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG64410.11808457686359958No Hit
GTCCTGCACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTC62530.11463792254744419No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA61370.11251126350130579No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG61000.11183293259865819No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA60940.11172293299282345No Hit
TATTTGGACTCAACTCTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATC60810.11148460051351482No Hit
CTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTGAG59850.1097246068201589No Hit
CTCTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG59590.10924794186154166No Hit
GTTCAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG58740.1076896141122161No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT58420.10710294954776411No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG56770.10407796038730863No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC55450.10165796905894421No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)