FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005107096

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107096
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2687739
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG117960.43888190036309327No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG97750.36368858732190884No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG86530.32194346251626366No Hit
GTAATAAAAGGCCGTGTCCGCAGCGGTCACAGAGTTCAGTTTCAGGGAGA84670.3150231477089107No Hit
ATCTATTACAGTGGGAGCACCACCTACAACCCCTCCCTCAAGAGTCGAGT84250.3134604959782181No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC79840.2970526528059458No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCAC64150.23867644886650083No Hit
CTATTATGCAAGCTGGTACCAGCAGAAGCCAGGACAGGCCCCTGTACTTG59720.22219419370705265No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT58370.2171713845726836No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAGATGGTTACCA56050.20853959406028638No Hit
GTAATAGATATACCCAATCCACTCCAGTCCCTTCCCTGGGGGCTGCCGGA55410.20615841047065953No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT53170.19782426790696567No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCT52440.19510823037504757No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA52310.19462455245840463No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA52240.1943641105032892No Hit
CCCCTGTACTTGTCATCTATGGTAAAAACAACCGGCCCTCAGGGATCCCA52080.1937688146058825No Hit
GTACAGGGGCCTGTCCTGGCTTCTGCTGGTACCAGCTTGCATAATAGCTT51600.1919829269136624No Hit
GTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAG50320.18722055973440874No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC49360.1836487843499685No Hit
GACTATTACTGTAACTCCCGGGACAGCAGTGGTAACCATCTGGTATTCGG47370.17624479162597262No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG46690.17371478406199412No Hit
CTATTACTGTAACTCCCGGGACAGCAGTGGTAACCATCTGGTATTCGGCG46250.1720777203441257No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC44760.16653402729952574No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG44080.16400401973554724No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC43240.16087871627416203No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC42800.1592416525562936No Hit
GTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCC42340.15753017685124934No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGA42130.15674885098590302No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG42010.156302379062848No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAG41920.15596752512055673No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA41720.15522340524879832No Hit
AGCCAGGACAGGCCCCTGTACTTGTCATCTATGGTAAAAACAACCGGCCC41200.15328869358222655No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA40850.15198648380664936No Hit
GGGTATATCTATTACAGTGGGAGCACCACCTACAACCCCTCCCTCAAGAG39930.14856353239656084No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG39840.14822867845426954No Hit
GTGGTAACCATCTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT39160.14569867089029104No Hit
CTATTACAGTGGGAGCACCACCTACAACCCCTCCCTCAAGAGTCGAGTCA38450.1430570453455488No Hit
TTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGT38230.14223851348661457No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG37840.14078747973668573No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAGATGGTTACCAC37780.14056424377515822No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT37450.1393364459867569No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA37230.1385179141278227No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT37190.138369090153471No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA36990.13762497028171264No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA36980.13758776428812472No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT36950.13747614630736094No Hit
ACCTACACCTGCGTGGTGGCCCATGAGGCCCTGCCCAACAGGGTCACCGA36940.13743894031377302No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT36030.13405319489727238No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC35780.1331230450575744No Hit
ACCTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTC35400.1317092173012335No Hit
CAGTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGA35340.131485981339706No Hit
GGTTACCACTGCTGTCCCGGGAGTTACAGTAATAGTCAGCCTCATCTTCC34780.1294024456987825No Hit
GTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCC34720.129179209737255No Hit
GTACTTGTCATCTATGGTAAAAACAACCGGCCCTCAGGGATCCCAGACCG34570.12862111983343621No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT34560.1285839138398483No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG34410.12802582393602951No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC34240.1273933220450349No Hit
GATATACCCAATCCACTCCAGTCCCTTCCCTGGGGGCTGCCGGATCCAGC33870.12601670028228187No Hit
GTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTG33130.12326345675677587No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC32700.12166359903249535No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT32410.12058462521844569No Hit
CTTTTATTACTGTGCGAGAGTTGGAACGGATAGCAGCAGCTGGTACTATT32390.12051021323126986No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC32140.11958006339157186No Hit
GTCCTGGCTTCTGCTGGTACCAGCTTGCATAATAGCTTCTGAGGCTGTCT32110.11946844541080812No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT32080.11935682743004436No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC31630.11768255771858802No Hit
CTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACA31460.11705005582759338No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC31400.11682681986606586No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA31050.1155246100904887No Hit
GCATAATAGCTTCTGAGGCTGTCTCCTTGGCATGTGATCCTGACTGTCTG30470.11336666246238938No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCC30390.11306901451368603No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG30340.11288298454574645No Hit
GTCCCAATAGTACCAGCTGCTGCTATCCGTTCCAACTCTCGCACAGTAAT30310.1127713665649827No Hit
CTGTAATAGATATACCCAATCCACTCCAGTCCCTTCCCTGGGGGCTGCCG30210.1123993066291035No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTG29350.10919959118054244No Hit
GTACAACGTGTCCCTGGTCATGTCCGACACAGCTGGCACCTGCTACTGAC29260.10886473723825119No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT29170.1085298832959599No Hit
ATGCAACATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAG29070.10815782336008073No Hit
GTCAGGATCACATGCCAAGGAGACAGCCTCAGAAGCTATTATGCAAGCTG28570.10629752368068476No Hit
CCCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACCAGT27650.10287457227059621No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG27550.10250251233471704No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCG27060.10067941864890899No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)