FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005107100

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107100
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3254112
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG158260.4863385156995211No Hit
GTAATACATGGCCGTGTCCGCGGCGGTCAGGGAGGTCAGCTTCAGGGAGA65760.2020827801870372No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51480.1581998406938667No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCC48170.14802809491498756No Hit
ATGTATTACTGTGCGAGAGAGGTGACTAGTGGGAGCCACTCGGACGCTTT46830.1439102280437797No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT39240.12058589255686343No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT37060.11388667630370436No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCACTGTCTGCGGCCC35270.10838594369216549No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA34950.1074025724990412No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT33910.10420661612138733No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA33680.10349981807632927No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC32640.10030386169867539No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)