FastQCFastQC Report
Sat 6 Aug 2022
EGAF00005107105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2619095
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA77360.2953692019571646No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC75000.2863584558788436No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC70890.27066601249668304No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT69000.26344977940853614No Hit
GTGTATTACTGTGCGAGGGCCGCCACAGTGACTAAGGTTATCTACTATTA67310.25699716886939955No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA65270.24920821886949499No Hit
GATTATTACTGCAGTTCATATGCAAGCAGCAGCACTCTGTATGTCTTCGG64390.2458482796538499No Hit
CTGTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAA61180.23359213774223536No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC60700.2317594436246108No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA60670.23164490024225926No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT60430.23072855318344693No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC60410.2306521909285459No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC60200.22985038725208515No Hit
TCATTAGACACGTCCAAGAACTTGTTCTCCCTGAAGCTGAGCTCTGTGAC58500.22335959558549803No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATACAGAGTGCTGC54430.20781987671313948No Hit
CTATTACACTGGGAGCACCAACTACAATCCCTCCCTCAAGAGTCGAGTCA54080.2064835372523715No Hit
TCTCTGGGCTCCGGGCTGAGGACGAGGCTGATTATTACTGCAGTTCATAT53020.20243633774261718No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATGACTATGTCTCCT52870.2018636208308595No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA51480.1965564441152383No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA51470.19651826298778774No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC50590.19315832377214268No Hit
GACTAAGGTTATCTACTATTACGGTATGGACGTCTGGGGCCAAGGGACCA50340.19220379558587986No Hit
CTATTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCT50080.1912110862721665No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT49580.1893020298996409No Hit
GTTCATATGCAAGCAGCAGCACTCTGTATGTCTTCGGAACTGGGACCAAG49550.18918748651728937No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT49480.18892021862513578No Hit
GGTTATGACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAA49270.188118414948675No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC48530.1852930115173371No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT48230.18414757769382173No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATACAGAGTGCTG47570.1816276232820879No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA47240.18036764607622097No Hit
TTTATGGGGTCACTAATCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGC46140.1761677220566646No Hit
TTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGG46010.17567136739980796No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATG45490.1736859487723813No Hit
GTAATAATCAGCCTCGTCCTCAGCCCGGAGCCCAGAGATGGTCAGGGAGG44450.169715111517528No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG44230.1688751267136167No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC43880.1675387872528488No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG43470.16597336102737778No Hit
CCGTAATAGTAGATAACCTTAGTCACTGTGGCGGCCCTCGCACAGTAATA43370.16559154975287266No Hit
GGATATATCTATTACACTGGGAGCACCAACTACAATCCCTCCCTCAAGAG43140.16471338382151088No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT42820.16349158774309447No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA42780.16333886323329241No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT40650.15520628308633327No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTG39760.15180816274323763No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG39590.15115908357657895No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC39300.15005183088051408No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG39170.14955547622365742No Hit
ACCTTAGTCACTGTGGCGGCCCTCGCACAGTAATACACGGCCGTGTCTGC38610.1474173330864287No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT38460.146844616174671No Hit
GTATTACTGTGCGAGGGCCGCCACAGTGACTAAGGTTATCTACTATTACG38070.14535555220410104No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC37550.14337013357667439No Hit
GACATACAGAGTGCTGCTGCTTGCATATGAACTGCAGTAATAATCAGCCT37170.1419192507335549No Hit
CTCTGTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCC37160.14188106960610442No Hit
ATCTATTACACTGGGAGCACCAACTACAATCCCTCCCTCAAGAGTCGAGT36350.13878839828261288No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC35900.13707024754733982No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT33150.12657043749844887No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACAC32870.12550136592983455No Hit
CATATGAACTGCAGTAATAATCAGCCTCGTCCTCAGCCCGGAGCCCAGAG32830.1253486414200325No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA32810.12527227916513148No Hit
GTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCCTCC32270.12321049828280378No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT31720.1211105362730256No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT30720.11729242352797435No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG30390.11603244632210745No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCT30380.11599426519465694No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA30120.11500155588094362No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT29840.11393248431232927No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA29730.11351249191037362No Hit
CAGCAGCACTCTGTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAG29340.11202342793980363No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC28670.1094652924006193No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT28660.10942711127316879No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG28460.10866348872415853No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGT28290.10801440955749983No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG27500.10499810048890934No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGA27060.1033181308810868No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGC27010.10312722524383423No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG26870.10259268945952704No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG26810.10236360269482397No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG26690.10190542916541781No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG26680.1018672480379673No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT26330.10053090857719937No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC26250.10022545955759529No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)