FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005107106

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107106
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2619095
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG102740.3922729034265653No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT81050.3094580379864037No Hit
CTATTACACTGGGAGCACCAACTACAATCCCTCCCTCAAGAGTCGAGTCA74620.28490757303572417No Hit
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA72860.278187694604434No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC68240.2605480137222972No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA64650.2468409889675632No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC63250.24149563112449146No Hit
GGATATATCTATTACACTGGGAGCACCAACTACAATCCCTCCCTCAAGAG62200.23748661274218766No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT60770.23202671151676435No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA59150.22584136886978134No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT59090.22561228210507828No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA58070.221717807105126No Hit
TCTCTGGGCTCCGGGCTGAGGACGAGGCTGATTATTACTGCAGTTCATAT57000.21763242646792116No Hit
GTGTATTACTGTGCGAGGGCCGCCACAGTGACTAAGGTTATCTACTATTA55840.21320341568366172No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC54980.20991983872291764No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA54720.20892712940920435No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT54290.20728534092883227No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATACAGAGTGCTGC54190.20690352965432715No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC53640.20480356764454896No Hit
ATCTATTACACTGGGAGCACCAACTACAATCCCTCCCTCAAGAGTCGAGT52200.19930548529167516No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT52040.19869458725246697No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC51510.19667098749758982No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATGACTATGTCTCCT50970.19460920661526213No Hit
TCATTAGACACGTCCAAGAACTTGTTCTCCCTGAAGCTGAGCTCTGTGAC50750.19376922181135087No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG50740.19373104068390035No Hit
GATTATTACTGCAGTTCATATGCAAGCAGCAGCACTCTGTATGTCTTCGG49690.18972202230159654No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATACAGAGTGCTG46950.17926039338015612No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT46820.17876403872329946No Hit
CTGTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAA46400.17716043137037793No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC45890.1752131938704018No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA45420.17341868088022772No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG44490.16986783602733005No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC44190.16872240220381468No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA43960.16784423627245287No Hit
GGTTATGACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAA42260.16135344460586576No Hit
CTATTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCT42180.16104799558626168No Hit
GACTAAGGTTATCTACTATTACGGTATGGACGTCTGGGGCCAAGGGACCA41670.15910075808628554No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC40250.15367903798831276No Hit
TTTATGGGGTCACTAATCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGC39500.15081545342952432No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT39470.1507009100471728No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC38390.14657734828251745No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATG38000.14508828431194745No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT37760.14417193725313515No Hit
GTTCATATGCAAGCAGCAGCACTCTGTATGTCTTCGGAACTGGGACCAAG37150.1418428884786539No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA36960.14111744705709414No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC36230.13833022475320675No Hit
TTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGG36010.13749023994929546No Hit
GTAATAATCAGCCTCGTCCTCAGCCCGGAGCCCAGAGATGGTCAGGGAGG35890.1370320664198893No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT35430.13527573455716574No Hit
GACATACAGAGTGCTGCTGCTTGCATATGAACTGCAGTAATAATCAGCCT35090.13397757622384832No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGT34930.1333666781846401No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACAC33480.1278304147043158No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT33450.12771587132196427No Hit
GGATTGGATATATCTATTACACTGGGAGCACCAACTACAATCCCTCCCTC33280.12706679215530556No Hit
GTATTACTGTGCGAGGGCCGCCACAGTGACTAAGGTTATCTACTATTACG32860.12546318480238403No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGG32130.12267596249849662No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT31870.12168325318478329No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTG31740.12118689852792662No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG31680.12095781176322355No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGT31170.11901057426324743No Hit
CCGTAATAGTAGATAACCTTAGTCACTGTGGCGGCCCTCGCACAGTAATA30640.11698697450837027No Hit
ACCTTAGTCACTGTGGCGGCCCTCGCACAGTAATACACGGCCGTGTCTGC30380.11599426519465694No Hit
CTACAATCCCTCCCTCAAGAGTCGAGTCACCATATCATTAGACACGTCCA30290.11565063504760233No Hit
GTGTAATAGATATATCCAATCCACTCCAGTCCCTTCCCTGGGGGCTGCCG30000.11454338235153745No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC29650.11320704289076952No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG29600.11301613725351696No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT29310.11190888455745211No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC29310.11190888455745211No Hit
GATATATCCAATCCACTCCAGTCCCTTCCCTGGGGGCTGCCGGATCCAGC29280.11179434117510056No Hit
GTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCCTCC29040.11087799411628825No Hit
CTCTGTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCC28790.10992346593002546No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC28580.10912166225356468No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG28470.10870166985160906No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC28230.10778532279279675No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT28150.10747987377319264No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG27700.10576172303791959No Hit
GTCTAATGATATGGTGACTCGACTCTTGAGGGAGGGATTGTAGTTGGTGC27160.10369994215559192No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA27140.10362357990069089No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG26740.1020963348026704No Hit
CATATGAACTGCAGTAATAATCAGCCTCGTCCTCAGCCCGGAGCCCAGAG26590.1015236178909127No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG26470.10106544436150654No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)