FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005107107

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107107
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4459856
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCT144730.324517204142914No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT127710.2863545370074729No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC122090.2737532332882497No Hit
GTCTACATGCAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTATATTA111580.2501874499983856No Hit
GTAATATACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTGCATGTAGA110820.24848335910397107No Hit
GTATATTACTGTGCGAGAGATTCCCAGGGGTTTTTTGGAGTCAACTACTA100560.22547813202937494No Hit
TTATTACTGCATGCAAGCTCTACAAACTCCTAAAACGTTCGGCCAAGGGA82090.18406423884537976No Hit
ATCCATTACTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC71530.1603863443124621No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT63280.1418879892086202No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC63020.14130501074474155No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCT61410.13769502871841602No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG61140.13708962800592664No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC59930.13437653592402984No Hit
GGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAATAATCAGC58390.13092350963797933No Hit
CATGTAGACTGTGCTCGTGGACGTGTCCCTGGTCATGGTGACTCTGTCCT58110.13029568667687924No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT57860.1297351304616113No Hit
GCATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGC56160.12592334819778933No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55920.12538521423113214No Hit
GTTTATTACTGCATGCAAGCTCTACAAACTCCTAAAACGTTCGGCCAAGG55130.12361385659088545No Hit
CCCTTATGATTTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG53400.11973480758123133No Hit
ATTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATG52180.11699929325072381No Hit
CAGTAATAAACTCCAATATCCTCAGCCTCCACTCTGCTGATTTTCAGTGT50110.11235788778830527No Hit
TTCCAGGACAGAGTCACCATGACCAGGGACACGTCCACGAGCACAGTCTA49490.1109677083744408No Hit
GTAATAAACTCCAATATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAA49150.1102053519216764No Hit
CTCCTAAAACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTG49070.11002597393279065No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT48580.1089272837508655No Hit
CTACTACGGTGTGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCT48240.10816492729810111No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG47730.10702139261895452No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG47200.10583301344258648No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC45200.101348563720443No Hit
TTTTAGGAGTTTGTAGAGCTTGCATGCAGTAATAAACTCCAATATCCTCA45140.1012140302287787No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCA44600.10000322880379994No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)