FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005107138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2758114
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAGGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC82820.3002776534980062No Hit
GATTATTACTGCTGCTCATATGCAGGTTCTAGCGCTTTCGCGTTATTTGG81110.2940777647334374No Hit
ATAATAGACGGCCGTGTCCGCAGCGGTCACAGAGCTCAGCTTCAGGGAGA79630.2887117791360328No Hit
GTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAACTGGATCCGGCATCC77590.2813154206098805No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGGCCAGAGATTGTCAGGGAGG74460.2699670862045586No Hit
GCCCTGGGCTGACCGACAACGGTCAGCTTGGTCCCTCCGCCAAATAACGC74120.26873435978353327No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG72320.2622081610839871No Hit
CTTATAACCTTGTCTCCTGGTACCGACAGCACCCAGGCAAAGCCCCCAAG70740.25647960889216326No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT57680.20912841166101184No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT52490.1903112054106538No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAAGCAGCAGTGATATT50600.18345869677613036No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA49800.1805581640207765No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA49680.18012308410747344No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT48990.17762137460598074No Hit
GTCTATTATTGTGCGAGAGAGGAGGCTTACTACTTTGACTACTGGGGCCA48560.17606233824997805No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA48450.1756635149961169No Hit
ATCTTTTACAGTGGGAGCACCAACTACAACCCCTCCCTGAAGAGTCGAGT46440.1683759264482904No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC45550.16514908375795925No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC45380.16453272054744655No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG44960.1630099408508858No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA44640.16184972774874426No Hit
CTCCTGCACTGGAAGCAGCAGTGATATTGGGACTTATAACCTTGTCTCCT42430.15383700601207928No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC41450.1502838533867708No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA39360.1427062115634089No Hit
CTATTATTGTGCGAGAGAGGAGGCTTACTACTTTGACTACTGGGGCCAGG38690.14027701538080006No Hit
GGGTATATCTTTTACAGTGGGAGCACCAACTACAACCCCTCCCTGAAGAG38100.1381378724737266No Hit
CAATAATAGACGGCCGTGTCCGCAGCGGTCACAGAGCTCAGCTTCAGGGA37890.13737648262544624No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC37620.13639755282051433No Hit
CTTTTACAGTGGGAGCACCAACTACAACCCCTCCCTGAAGAGTCGAGTCA36890.13375081668125394No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG36560.13255434691967047No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC36250.13143039047697086No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT36170.13114033720143548No Hit
GTGATATTGGGACTTATAACCTTGTCTCCTGGTACCGACAGCACCCAGGC36110.13092279724478392No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG35940.13030643403427125No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA35820.12987135412096817No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA35810.12983509746152624No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG34650.1256293249662632No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT34550.12526675837184395No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT34310.1243965985452378No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCCTGGGCTGA33770.12243873893537395No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT33550.12164109242765164No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT33310.12077093260104549No Hit
TCCCTGAAGAGTCGAGTCACCATGTCAGCAGACACGTCCAAGAATCAGTT33090.11997328609332318No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC33080.11993702943388126No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA32870.11917563958560087No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA32740.11870430301285588No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAACTGGA32230.11685521338131782No Hit
TATTGGGACTTATAACCTTGTCTCCTGGTACCGACAGCACCCAGGCAAAG32230.11685521338131782No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC31090.1127219542049386No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC30670.11119917450837782No Hit
GTCGATCACCATCTCCTGCACTGGAAGCAGCAGTGATATTGGGACTTATA30430.11032901468177167No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT30020.10884249164465284No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG29720.10775479186139515No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC29530.1070659153319986No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT29220.105941958889299No Hit
CTACAACCCCTCCCTGAAGAGTCGAGTCACCATGTCAGCAGACACGTCCA28750.1042378958955286No Hit
CTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCC28280.10253383290175823No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA28190.10220752296678091No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG27990.10148238977794245No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT27730.10053971663245245No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)