FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005107167

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005107167
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4231900
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGTGTCCTCAGATTTCAGGCTGCTCAGCTCCATGTAGA117720.2781729246910371No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG115010.2717691816914389No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG111450.26335688461447576No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC101180.23908882535031545No Hit
GTCTATTACTGTGCGAGAGATTGTGGTGGTGGTGACTTCATTATTTTGGG85090.2010680781681987No Hit
CTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGG75720.17892672322124814No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT74220.17538221602589854No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA72060.1702781256645951No Hit
GTCTACATGGAGCTGAGCAGCCTGAAATCTGAGGACACGGCCGTCTATTA70160.1657884165504856No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT68960.1629528107942059No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA66500.15713981899383256No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT65840.15558023582787872No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCACATGAATGCCA64340.15203572863252912No Hit
GGTTAACAGTGATGGCAGCCACAACAAGGGGGACGGGATCCCTGATCGCT61800.1460336964484038No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG58950.13929913277723951No Hit
GACTATTACTGTCAGACCTGGGGCACTGGCATTCATGTGGTGTTCGGCGG57510.13589640586970392No Hit
CTATTACTGTCAGACCTGGGGCACTGGCATTCATGTGGTGTTCGGCGGAG56020.1323755287223233No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG55220.13048512488480352No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC54210.1280984900399348No Hit
GTACTTGATGAAGGTTAACAGTGATGGCAGCCACAACAAGGGGGACGGGA53260.12585363548288003No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG52370.12375056121363927No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT51900.12263994895909638No Hit
ACTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATG50840.12013516387438267No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT50440.11918996195562277No Hit
GTTCTGGGGCTGAGCGCCACCTCACCATCTCCAGCCTCCAGTCTGAGGAT50220.11867010090030483No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT50200.11862284080436684No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT49980.11810297974904889No Hit
GTAATAGTCAGCCTCATCCTCAGACTGGAGGCTGGAGATGGTGAGGTGGC49960.11805571965311089No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA49580.11715777783028901No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG49330.11656702663106405No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA48980.11573997495214915No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCAC48890.11552730452042818No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCA48830.11538552423261418No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG48390.11434580212197831No Hit
ATTCATATCTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAGGGAGTGC48390.11434580212197831No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA47890.11316429972352843No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC47600.11247902833242751No Hit
CTACAGAAGCCTCTGAGAGGAAAGTTCTTCACCATGGACTGGACCTGGAG47110.11132115598194664No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46580.11006876343958977No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACT46360.10954890238427184No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG45980.10865096056144993No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA45640.10784753893050403No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG45440.10737493797112409No Hit
GATATGAATACCTCCCAAAATAATGAAGTCACCACCACCACAATCTCTCG44620.1054372740376663No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA44340.10477563269453438No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT43260.10222358751388265No Hit
ACCTACACCTGCGTGGTGGCCCATGAGGCCCTGCCCAACAGGGTCACCGA42560.10056948415605284No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)